Transcript Powerpoint - Blood Journal

A novel I-branching β-1,6-N-acetylglucosaminyltransferase
involved in human blood group I antigen expression
by Niro Inaba, Toru Hiruma, Akira Togayachi, Hiroko Iwasaki, Xiao-Hui Wang,
Yusuke Furukawa, Ryoichi Sumi, Takashi Kudo, Katsuya Fujimura, Toshie Iwai,
Masanori Gotoh, Mitsuru Nakamura, and Hisashi Narimatsu
Blood
Volume 101(7):2870-2876
April 1, 2003
©2003 by American Society of Hematology
Structures of the I and i antigens and their biosynthetic pathways.The structure of the i antigen
determinant is a linearN-acetyllactosamine unit repeated, and that of the I antigen determinant is
a branched poly-N-acetyllactosamine.
Niro Inaba et al. Blood 2003;101:2870-2876
©2003 by American Society of Hematology
Genomic organization and multiple amino acid alignments of IGnT and novel IGnT-homologous
sequences.(A) Two BAC clones containing novel IGnT-homologous sequences. 2 BAC clones,
RP11-360O19 and RP11-421M1, are illustrated.
Niro Inaba et al. Blood 2003;101:2870-2876
©2003 by American Society of Hematology
Quantitative analysis of IGnT transcripts in human tissues by real-time PCR.Standard curve for
IGnTs was generated by serial dilution of each plasmid DNA. The expression level of IGnT
transcripts was normalized to micrograms of mRNA.
Niro Inaba et al. Blood 2003;101:2870-2876
©2003 by American Society of Hematology
Changes of IGnT transcript expression during hematopoietic cell differentiation from CD34positive cells.The expression level of IGnTs was analyzed by real-time PCR. Standard curve for
IGnTs was generated by serial dilution of each plasmid DNA. The expressi...
Niro Inaba et al. Blood 2003;101:2870-2876
©2003 by American Society of Hematology
Segregation of wild-type I3,I3i1 , and I3i3 alleles in an adult i pedigree.(A) Pedigree drawings I, II,
and III represent the first, second, and third generation, respectively, and the propositus is
indicated by an arrow.
Niro Inaba et al. Blood 2003;101:2870-2876
©2003 by American Society of Hematology
Flow cytometric analyses of CHO cells transfected withIGnT3 cDNAs containing the 1049G>A
mutation or the 1006G>A mutation.CHO cells transfected with the vector alone (A-C), pcDNA3.1IGnT3 [1049G>A] (D-F), pcDNA3.1-IGnT3[816G>C/1006G>A] (G-I), and wild-type ...
Niro Inaba et al. Blood 2003;101:2870-2876
©2003 by American Society of Hematology