Transcript Powerpoint - Blood Journal

Pleiotrophin produced by multiple myeloma induces
transdifferentiation of monocytes into vascular endothelial
cells: a novel mechanism of tumor-induced
vasculogenesis
by Haiming Chen, Richard A. Campbell, Yunchao Chang, Mingjie Li, Cathy S. Wang,
Jennifer Li, Eric Sanchez, Michael Share, Jeffrey Steinberg, Ariana Berenson, Dror
Shalitin, Zhaohui Zeng, Dorina Gui, Pablo Perez-Pinera, Ronald J. Berenson,
Jonathan Said, Benjamin Bonavida, Thomas F. Deuel, and James R. Berenson
Blood
Volume 113(9):1992-2002
February 26, 2009
©2009 by American Society of Hematology
PTN and M-CSF together induce VEC gene expression in fresh monocytes in vitro.
Haiming Chen et al. Blood 2009;113:1992-2002
©2009 by American Society of Hematology
MM tumor cells stimulate tube-like structure formation and Flk-1 staining in freshly obtained
CD14+ cells that are blocked by anti-PTN antibodies; PTN and M-CSF induce tube-like structure
formation and Flk-1 staining in fresh human monocytes in vitro that i...
Haiming Chen et al. Blood 2009;113:1992-2002
©2009 by American Society of Hematology
MM cells as well as PCL serum induce VEC gene and protein expression in monocytes that is
blocked by anti-PTN antibodies.
Haiming Chen et al. Blood 2009;113:1992-2002
©2009 by American Society of Hematology
CD133 and CD45 expression in CD14+ cells alone or treated with PTN, M-CSF, or the
combination of both cytokines for 7 days.
Haiming Chen et al. Blood 2009;113:1992-2002
©2009 by American Society of Hematology
To determine whether PTN induced the monocyte cell line THP-1 to express VEC genes, total
RNA was isolated and separated by 1% agarose gel electrophoresis after THP-1 monocytes were
cultured with PMA, PCL serum, or normal human serum or cocultured with U266...
Haiming Chen et al. Blood 2009;113:1992-2002
©2009 by American Society of Hematology
GFP-marked THP-1 cells (THP-1/GFP) coinjected with human LAGλ-1 MM tumor cells into C.B-17
SCID/SCID mice become incorporated into tumor blood vessels and express VEC markers.
Haiming Chen et al. Blood 2009;113:1992-2002
©2009 by American Society of Hematology
Microdissection of single cells lining blood vessels from LAGλ-1 tumors containing THP-1 cells
shows cells with the presence of 3 different types of DNA: only human, both human and mouse,
and only murine sequences.
Haiming Chen et al. Blood 2009;113:1992-2002
©2009 by American Society of Hematology