Transcript Digesting Macromolecules

Digesting macromolecules lab
Outline of the day
1. Turn in your lab reports at the front
–
More than 10 minutes late = bad
Any questions on last week’s lab?
Quiz
Introduction to the lab
Lab!
Check out
2.
3.
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6.
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Get a stamp
Make sure I mark you down for attendance
Quiz
• Ends 10 minutes after it’s started
– Ends at: ____
Lab this week!
• Exploring digestion!
– How carbohydrates, proteins, and fats are
digested
– Effect of enzyme inhibitors on carbohydrate
digestion
– Effect of pH on protein digestion
– Requirements for fat digestion
Why do we eat?
What are we doing when we
eat?
• Digestion: Dissolving food and breaking
it down into smaller molecules
• Absorption: The entry of molecules
across the gut epithelium into the body
What types of macromolecules are
there?
• How do we digest them?
Digesting food
• Dozens of enzymes
help during digestion
– Enzymes make
reactions go faster
(and be more likely to
occur)
Missing image:
Image showing enzymes
reducing the energy require
for a reaction to progress.
Today we’re going to see what effects
the digestion of macromolecules
• Using indicators to test for various steps of
digestion
– E.g., Iodine changes color in the presence of
starch
• Blue/black = starch present
• Brown = no starch present
Detecting the breakdown of starch
with iodine
Missing image:
IKI test samples
Detecting the breakdown of starch
by testing for the presence of sugar
Missing image:
Benedict’s test
Detecting the breakdown of protein
into amino acids with egg whites
Missing image:
Egg white test
Detecting the breakdown of fat into
glycerol and fatty acids
Missing text:
Description of lipid test
Before you leave
• Clean up your work area
– Wash glassware and store upside down
• Show me your lab report so I can stamp it
– Need to have all data fields filled in
– Complete at home and then turn in at the beginning of
next lab
• Remember that we’ll have a quiz at the
beginning of the next class
– 6-7 questions on today’s lab
– 3-4 questions on the lab we’ll do next week
Notes for the instructor:
• Add any relevant cleanup instructions to the final
slide (that slide is a generic one I’m adding to
each presentation).
• The last few slides (on each biochemical test)
were based on excellent slides from Kelli Elliott
(of Orange Coast College); contact her for the
images and other text that go along with them.
• Note also that the first few slides of the
presentation are designed to be used to facilitate
class discussion (I create lists of the answers to
each question on the board as the class
provides them)
License information
• This work is licensed under the Creative Commons AttributionNonCommercial-ShareAlike 3.0 License. To view a copy of this
license, visit http://creativecommons.org/licenses/by-ncsa/3.0/us/ or send a letter to Creative Commons, 171 Second
Street, Suite 300, San Francisco, California, 94105, USA.
• The slides in this presentation were originally created by Marc
C. Perkins (http://faculty.orangecoastcollege.edu/mperkins).
• You are free to use, modify, and distribute these slides
according to the terms of the Creative Commons license (e.g.,
you must attribute the slides, no commercial uses are allowed,
and future distributions must be licensed under a similar
license).
• Attribution should be given to Marc C. Perkins (and any later
editors), including a link back to Marc’s current website. This
applies both while distributing the slides and during use of the
slides; attribution during use can be satisfied by, for instance,
placing small text on at least one of the slides that has been
shown (see below for an example).
History
• August 2007: Marc Perkins released first
version.
http://faculty.orangecoastcollege.edu/mperkins
(If you modify these slides and redistribute them, add your information to the list)