Transcript lab 10 - the circulatory system physiol Lecture Notes Page

Laboratory Ten
The Circulatory System:
Physiology
Circle of Willis
(circulation to the brain)
Hepatic Portal Circulation
(shunts blood from digestive organs to the liver for final
metabolism and detoxification and from liver to the inferior
vena cava for return to the heart)
Fetal Circulation
(designed to by-pass fetal lung)
Lymphatic System
(related to body defense mechanisms)
The Electrocardiogram: Recording of Heart
Electrical Activity
•P wave
•Atrial depolarization
•Occurs before atrial
contraction
•Generated by the firing of
Sinoarterial (SA) node
•QRS complex
•Ventricular depolarization
•Occurs before ventricular
contraction (systole)
•T wave
•Ventricular repolarization
•Occurs before ventricular
relaxation (diastole)
Pulse & Blood Pressure
•Determine pulse rate manually
in 5 different locations
•Measure systemic blood
pressure using the peripheral
pulses in the brachial artery
•Systolic pressure (first soft
sound): generated by
contraction of left ventricles
•Diastolic pressure (sound
terminates): corresponds to
relaxation of the left
ventricle
Blood Typing
Anti-Serum
Agglutination
Antigen
Anti-A
Yes
‘A’ Present
Anti-A
No
Anti-B
Yes
‘A’ NOT
Present
‘B’ Present
Anti-B
No
Anti-Rh
Yes
Anti-Rh
No
‘B’ NOT
Present
‘Rh’ Present
‘Rh’ NOT
Present
Blood Typing
• 2 drops of the synthetic blood of only
one subject
• 2 drops of each anti-serum
• Mix with a separate toothpicks (avoid
cross contamination)
Anti-B
• Place the slide on the Rh-typing box
for 30-45sec while gently rocking the
box
• Observe the wells for agglutination
(RBC clumping)
• Record your results in your handout
• e.g.:
Anti-A
– B- (agglutination in anti-B well)
– O+ (agglutination in anti-Rh well)
– AB+ (agglutination in Anti-A, -B,&
-Rh wells)
B
Rh
A
Anti-Rh