Transcript Chapter 16 Worksheets

Chapter 16 Worksheets
Recombinant DNA lab
Mini Labs
Reinforcement & Study Guides
Content Mastery Crossword
Term
Model
Gene Splicing
process of taping green & red papers together
Plasmid
green strip
Restriction Enzyme
scissors
Sticky Ends
cut ends on paper
Recombinant DNA
red & green strips taped together
Analyze & Conclude:
1. Comparing & Contrasting: How does the paper model of a plasmid resemble a bacterial
plasmid?
It is a small circular piece of DNA.
2. Comparing & Contrasting: How is cutting with the scissors different from cleaving with a
restriction enzyme?
The scissors can cut the DNA anywhere, but the restriction enzyme recognizes a particular
sequence.
3. Thinking Critically: Some restriction enzymes cut DNA at particular places but do not leave
sticky ends. These enzymes cannot be used to engineer recombinant DNA. Explain why.
What function might they serve in a cell?
Without the sticky ends, the pieces of DNA can’t be inserted into other DNA. These enzymes
may function in a cell by cutting invading viral DNA.
Expected results: Students will examine how a specific
restriction enzyme cuts a strand of DNA
Bacteria use restriction enzymes to cut up viral DNA
The ends join together with complimentary
single strands on other split DNA molecules
The cut below does not produce sticky ends, so the DNA
fragment would not combine readily with other DNA fragments.
Expected Results: Students will splice together the two
different colors of wire, representing the insertion of a
gene from a different species into a strand of DNA
The wires represent DNA segments from two different
species and the pieces of wire represent genes
Stripping the wire is like using restriction enzymes
to form sticky ends
Splicing the wires together now allows the wires to function
as a unit, and splicing a gene into a fragmented piece of DNA
allows that gene to function within the DNA strand
c
e
a
b
d
f
√
√
√
√
DNA fragment
plasmid (vector)
gene splicing
Recombinant DNA
bacterial cell (host)
plasmid containing
bacterium
cell division
clone
How is cloning possible?
What do genetic-engineering techniques provide?
√
√
√
√
√
√
√
√
√
√
√
√
√
100,000
have not
linkage map
sperm
cystic fibrosis
Electrophoresis
It is an international effort to completely
map & sequence the human genome.
Because humans have only a few children compared
to other species and the generation time is so long
Prenatal diagnosis of human disorders, gene therapy, and DNA fingerprinting
Because no two individuals with the exception of identical
twins have the same DNA sequence, and all autosomal
cells of an individual have the same DNA
clones
human genome
recombinant DNA
plasmid
cell culture
vector
transgenic
organism
gene therapy
restriction enzyme
linkage map
genetic engineering
gene splicing
Location A represents the longest fragment; location F represents
the shortest fragment. Because smaller fragments of DNA move
more quickly through the gel, the farther the standard marker
band is from the well, the smaller is the fragment
Father #2 might be the biological father because he matches a
band with the child at F. Suspected father #1 does not match any
bands with the child
It would mean that the person is homozygous for the segment of DNA
containing the repeated sequence. If the person is heterozygous, a
separate band will be seen for each of the two alleles. However, if he or
she is homozygous, there will be only one band visible
Any combination of one band
from the mother & one band from
the father would be correct.