Transcript Document

Ligase: joins (seals) the
ends of DNA
Vectors: DNA molecule that can
replicate & is used to transport
foreign DNA
Four types of Vectors: Plasmids; Bacteriophage & other viruses; artificial
chromosomes (YAC or yeast artificial chromosome/ DNA + Ori +centromere for
sister chromatid separation + telomeres to mark end of chromosome); & Cosmids
(plasmid + l cos site & can be pckged into phage capsid)
Ti sequence 23 bp direct
repeats, that flank the
virulence & hormone
genes
Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti
(causes crown gall tumors). Plasmid codes for virulence gene and regulates
pant growth (codes for plant growth hormones, auxin & cytokinin). It acts
like a transposon.