Transcript ppt - ResistVir

Expert Group 2
"Mechanisms and sources of resistance"
Boulton lab (JIC):
Mastrevirus genus of the Geminiviridae
(mainly infect cereals e.g. Wheat dwarf virus (WDV) and
Maize streak virus (MSV))
WDV: Europe (Scandinavia →France →Czech Rep., Hungary)
MSV: Sub-Saharan Africa and Indian Ocean islands
Interests:
Mastrevirus-host interactions (susceptible and resistant)
a) Interaction of virus early gene products with host proteins
(modulation of host cell cycle, virus replication)
a) characterisation of resistance in cereals
Interaction of geminivirus early gene products
with host proteins
Bean yellow dwarf virus and Beet curly top virus + Arabidopsis
Identification of pathways involved (e.g. microarray analysis of
transgenic plants inducibly expressing Rep, RepA and C4)
Identification of susceptibility factors – candidate genes and
genes identified in suppressor screens following ems
mutagenesis
Basis for geminivirus resistance via TILLING populations
Biological and genetic characterisation of
resistance to geminiviruses (WDV) in cereals
Biological characterisation:
Identification of resistant/tolerant cultivars:
a) Vector transmission: Psammotettix alienus + WDV-[CZ] (wheat)
and WDV-[HU] (barley)
Established colony and conditions
JIC insectary, with Ian Bedford)
H Inf
WDV-[HU] in barley
Psammotettix alienus
Insect collection
Biological and genetic characterisation of
resistance to geminiviruses (WDV) in cereals
Biological characterisation:
b) Agroinoculation:
Mechanism for inoculation of cloned DNA
Established conditions for inoculation of seedlings
at different developmental stages (barley and wheat)
Seed inoculation (barley)
Seedling inoculation (wheat)
WDV-[CZ] in wheat
Two techniques: Differentiate between resistance to
virus replication/insect transmission
Biological and genetic characterisation of
resistance to geminiviruses in cereals
Genetic characterisation:
(of HvMsv1, with Dave Laurie)
Map based cloning:
Using doubled haploid and F2, F3 populations
barley cvs: Igri x Triumph
Transcript based cloning:
Bulked segregant approach
Candidate genes
Triumph
Igri
Potential for ResistVir
(JIC Boulton group)
[WDV, wheat and barley]
1. Methods for screening susceptibility of germplasm: cloned
and natural isolates (agroinoculation and vector transmission)
2. Mapping technologies for identification genes/markers: wheat
and barley)
Improved resistance to WDV in cereals
Collaborative potential/synergy/overlap?
WDV:
Partner 22 (Aschersleben, Germany)
Partner 26 (NIAB –Kenyon)
Susceptibility factors:
Partner 39
Assist further progress?
1. Information: Availability of germplasm: RILs, doubled haploids,
mutant populations
2. Information/access: Tilling populations produced
3. Access to high throughput marker technology