Transcript ch 20 biotech clicker questions

CLICKER QUESTIONS
For CAMPBELL BIOLOGY, NINTH EDITION
Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Chapter 20
Biotechnology
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Questions prepared by
Christopher Gregg
Louisiana State University
Louise Paquin
McDaniel College
© 2011 Pearson Education, Inc.
Which of the following is an example of
"recombinant DNA technology?"
a) combining alternate alleles of a gene in a single
cell
b) manipulating a meiotic crossing-over event
c) cloning genes from homologous pairs of
chromosomes
d) introducing a human gene into a bacterial
plasmid
e) alternate alleles assorting independently
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How does a bacterial cell protect its own DNA
from restriction enzymes?
a) by forming "sticky ends" of bacterial DNA to prevent the
enzyme from attaching
b) by using DNA ligase to seal the bacterial DNA into a
closed circle
c) by reinforcing the bacterial DNA structure with covalent
phosphodiester bonds
d) by adding histones to protect the double-stranded DNA
e) by adding methyl groups to adenines and cytosines
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One strand of a DNA molecule has the
sequence 3′-GGATGCCCTAGGCTTGTT-5′
Which of the following is the complementary
strand?
a) 3′-AACAAGCCTAGGGCATCC-5′
b) 3′-CCTACGGGATCCGAACAA-5′
c) 5′-AACAAGCCTAGGGCATCC-3′
d) 5′-GGATGCCCTAGGCTTGTT-3′
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Which of the following DNA molecules is most
likely to be cut by a restriction enzyme?
a) 5′-AAGCCT-3′
b) 5′-GGAAGG-3′
c) 5′-GGATCC-3′
d) 5′-AATTAA-3′
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You have a restriction enzyme that makes a
blunt cut between an A and a T. What will the size
of the DNA fragments be after the following DNA
molecule is cut with this restriction enzyme:
5′-TTGTTCGGATCCCGTAGG-3′?
a) one 9-bp fragment, one 6-bp fragment, and one 3bp fragment
b) one 15-bp fragment and one 3-bp fragment
c) one 18-bp fragment
d) two 9-bp fragments
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You have isolated this eukaryotic gene and wish
to express the protein it codes for in a culture of
recombinant bacteria. Will you be able to produce
a functioning protein with the gene as is?
a) yes
b) No, the exons will need to be cut out and the introns spliced
back together.
c) No, the introns will need to be cut out and the exons spliced
back together.
d) No, the exons will need to be cut out, the introns translated
individually, and the peptides bound together after translation.
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This segment of DNA is cut at restriction sites
1 and 2, which creates restriction fragments A,
B, and C. Which of the following
electrophoretic gels represents the separation
of these fragments?
a) Answer a
b)
c) Answer
d)
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A dideoxy chain-terminating sequencing
reaction produces the following batch of
labeled DNA strands. What is the sequence of
the template DNA strand?
a) 5′-AGGCTT-3′
b) 5′-AAGCCT-3′
c) 5′-TCCGAA-3′
d) 5′-TTCGGA-3′
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The photograph shows Rainbow and CC (CC
is Rainbow’s clone). Why is CC’s coat pattern
different from Rainbow’s given that CC is
genetically identical?
a) X chromosome
inactivation
b) heterozygous at coat
color gene locus
c) environmental effects
on gene expression
d) all of the above
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The first cell whose entire genome was
sequenced was which of the following?
a) H. influenzae in 1995
b) H. sapiens in 2001
c) Rice in 1955
d) Tobacco mosaic virus
e) HIV in 1998
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For discussion:
Why has gene therapy had only rather limited
success?
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