Transcript C9 Challenge

Chapter 9: Biotechnology and DNA Technology
Biotechnology
Tools of
Biotechnology
Recombinant
DNA
Applications
of rDNA
Products of
Genetic
Engineering
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FINAL ROUND
© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$100 Question
Today, chemicals that organisms do NOT
naturally make are made by microorganisms
and
a.
b.
c.
d.
plants.
fungi.
protozoa.
helminths.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$100 Answer
Today, chemicals that organisms do NOT
naturally make are made by microorganisms
and
a.
b.
c.
d.
plants.
fungi.
protozoa.
helminths.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$200 Question
When E. coli has the ability to produce the
hormone insulin, what is the reason?
a.
b.
c.
d.
It picked up the insulin gene from another cell.
It is an ancient gene that now has no function.
The insulin gene was inserted into it.
It needs to regulate its cell glucose level.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$200 Answer
When E. coli has the ability to produce the
hormone insulin, what is the reason?
a.
b.
c.
d.
It picked up the insulin gene from another cell.
It is an ancient gene that now has no function.
The insulin gene was inserted into it.
It needs to regulate its cell glucose level.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$300 Question
Natural selection is the process
a. of inserting genes into cells by rDNA technology.
b. whereby organisms with traits that make them better
adapted to their environment tend to survive and
reproduce more than organisms lacking these traits.
c. by which scientists select desirable breeds of animals or
strains of plants to cultivate.
d. None of the answers is correct.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$300 Answer
Natural selection is the process
a. of inserting genes into cells by rDNA technology.
b. whereby organisms with traits that make them better
adapted to their environment tend to survive and
reproduce more than organisms lacking these traits.
c. by which scientists select desirable breeds of animals or
strains of plants to cultivate.
d. None of the answers is correct.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$400 Question
In the field of genetic engineering, cDNA is
important because it
a.
b.
c.
d.
lacks exons.
lacks introns.
is really RNA.
is really reverse transcriptase.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$400 Answer
In the field of genetic engineering, cDNA is
important because it
a.
b.
c.
d.
lacks exons.
lacks introns.
is really RNA.
is really reverse transcriptase.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$500 Question
The procedure during which cells can take up
DNA from the surrounding environment is
called
a.
b.
c.
d.
electroporation.
protoplast fusion.
transformation.
microinjection.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Biotechnology
$500 Answer
The procedure during which cells can take up
DNA from the surrounding environment is
called
a.
b.
c.
d.
electroporation.
protoplast fusion.
transformation.
microinjection.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$100 Question
Which technique amplifies small samples of
DNA to quantities that are large enough for
analysis?
a.
b.
c.
d.
hybridization
PCR
RFLP
DNA fingerprinting
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$100 Answer
Which technique amplifies small samples of
DNA to quantities that are large enough for
analysis?
a.
b.
c.
d.
hybridization
PCR
RFLP
DNA fingerprinting
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$200 Question
Which of the following techniques is used to
identify bacteria carrying a specific gene?
a.
b.
c.
d.
cloning
Western blot
transformation
DNA probe
ANSWER
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© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$200 Answer
Which of the following techniques is used to
identify bacteria carrying a specific gene?
a.
b.
c.
d.
cloning
Western blot
transformation
DNA probe
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$300 Question
If a restriction enzyme made a staggered cut
in DNA, the ends would be termed _____
ends.
a.
b.
c.
d.
flat
sticky
adhesive
blunt
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$300 Answer
If a restriction enzyme made a staggered cut
in DNA, the ends would be termed _____
ends.
a.
b.
c.
d.
flat
sticky
adhesive
blunt
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$400 Question
A population of genetically identical cells
carrying a desired vector is called
a.
b.
c.
d.
a clone.
a species.
a library.
PCR.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$400 Answer
A population of genetically identical cells
carrying a desired vector is called
a.
b.
c.
d.
a clone.
a species.
a library.
PCR.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$500 Question
Which of the following methods is especially
valuable in the genetic manipulation of plant
and algal cells?
a.
b.
c.
d.
protoplast fusion
viral transduction
transformation
cloning
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Tools of Biotechnology
$500 Answer
Which of the following methods is especially
valuable in the genetic manipulation of plant
and algal cells?
a.
b.
c.
d.
protoplast fusion
viral transduction
transformation
cloning
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$100 Question
Which of the following is NOT a method for
inserting foreign DNA into cells?
a.
b.
c.
d.
transformation
microinjection
restriction digestion
electroporation
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$100 Answer
Which of the following is NOT a method for
inserting foreign DNA into cells?
a.
b.
c.
d.
transformation
microinjection
restriction digestion
electroporation
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$200 Question
Which of the following is a eukaryote used in
genetic engineering?
a.
b.
c.
d.
Agrobacterium tumefaciens
Pseudomonas aeruginosa
Saccharomyces cerevisiae
Thermus aquaticus
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$200 Answer
Which of the following is a eukaryote used in
genetic engineering?
a.
b.
c.
d.
Agrobacterium tumefaciens
Pseudomonas aeruginosa
Saccharomyces cerevisiae
Thermus aquaticus
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$300 Question
What are the two main sources of DNA that
scientists use to obtain the genes in which
they are interested?
a.
b.
c.
d.
genomic libraries and PCR
synthetic DNA and plasmids
synthetic DNA and genomic libraries
PCR and plasmids
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$300 Answer
What are the two main sources of DNA that
scientists use to obtain the genes in which
they are interested?
a.
b.
c.
d.
genomic libraries and PCR
synthetic DNA and plasmids
synthetic DNA and genomic libraries
PCR and plasmids
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$400 Question
Restriction enzymes are
a.
b.
c.
d.
animal enzymes that splice RNA.
bacterial enzymes that destroy phage DNA.
animal enzymes that destroy RNA.
viral enzymes that destroy host DNA.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$400 Answer
Restriction enzymes are
a.
b.
c.
d.
animal enzymes that splice RNA.
bacterial enzymes that destroy phage DNA.
animal enzymes that destroy RNA.
viral enzymes that destroy host DNA.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$500 Question
Which enzyme is used to synthesize cDNA?
a.
b.
c.
d.
RNA polymerase
complementary polymerase
reverse transcriptase
DNA polymerase
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Recombinant DNA
$500 Answer
Which enzyme is used to synthesize cDNA?
a.
b.
c.
d.
RNA polymerase
complementary polymerase
reverse transcriptase
DNA polymerase
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$100 Question
In plants, the Ti plasmid can cause
a.
b.
c.
d.
crown gall disease.
tobacco mosaic disease.
Pseudomonas.
soft rot disease.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$100 Answer
In plants, the Ti plasmid can cause
a.
b.
c.
d.
crown gall disease.
tobacco mosaic disease.
Pseudomonas.
soft rot disease.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$200 Question
Molecule-sized robots used to detect
contamination in food are an example of
a.
b.
c.
d.
forensic microbiology.
nanotechnology.
biotechnology.
bioinformatics.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$200 Answer
Molecule-sized robots used to detect
contamination in food are an example of
a.
b.
c.
d.
forensic microbiology.
nanotechnology.
biotechnology.
bioinformatics.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$300 Question
What can be used to screen a sample for
multiple pathogens simultaneously?
a.
b.
c.
d.
DNA chips
Southern blotting
shotgun sequencing
colony hybridization
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$300 Answer
What can be used to screen a sample for
multiple pathogens simultaneously?
a.
b.
c.
d.
DNA chips
Southern blotting
shotgun sequencing
colony hybridization
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$400 Question
When mammalian cells are used to make
protein products in genetic engineering, there
is a(n)
a.
b.
c.
d.
high risk of toxin production.
increase in allergic reactions.
low risk of toxin production.
low rate of protein production.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$400 Answer
When mammalian cells are used to make
protein products in genetic engineering, there is
a(n)
a.
b.
c.
d.
high risk of toxin production.
increase in allergic reactions.
low risk of toxin production.
low rate of protein production.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$500 Question
Which bacterium has been genetically modified to
produce a human protein, gamma interferon?
a.
b.
c.
d.
Bacillus thuringiensis
Staphylococcus aureus
Escherichia coli
Pseudomonas fluorescens
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Applications of rDNA
$500 Answer
Which bacterium has been genetically modified to
produce a human protein, gamma interferon?
a.
b.
c.
d.
Bacillus thuringiensis
Staphylococcus aureus
Escherichia coli
Pseudomonas fluorescens
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$100 Question
The Ti plasmid naturally occurs in
a.
b.
c.
d.
Thermus aquaticus.
Saccharomyces cerevisiae.
Bacillus thuringiensis.
Agrobacterium tumefaciens.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$100 Answer
The Ti plasmid naturally occurs in
a.
b.
c.
d.
Thermus aquaticus.
Saccharomyces cerevisiae.
Bacillus thuringiensis.
Agrobacterium tumefaciens.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$200 Question
The method of using RFLPs to identify
bacterial or viral pathogens is called
a.
b.
c.
d.
proteomics.
DNA fingerprinting.
genetic screening.
DNA sequencing.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$200 Answer
The method of using RFLPs to identify
bacterial or viral pathogens is called
a.
b.
c.
d.
proteomics.
DNA fingerprinting.
genetic screening.
DNA sequencing.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$300 Question
One of the earliest commercial products of rDNA
technology was
a.
b.
c.
d.
rennin.
cellulase.
malaria vaccine.
human growth hormone.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$300 Answer
One of the earliest commercial products of rDNA
technology was
a.
b.
c.
d.
rennin.
cellulase.
malaria vaccine.
human growth hormone.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$400 Question
Which of the following is NOT a therapeutic
application of biotechnology?
a.
b.
c.
d.
creating herbicide-resistant plants
producing DNA vaccines
gene therapy
synthesizing subunit vaccines
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$400 Answer
Which of the following is NOT a therapeutic
application of biotechnology?
a.
b.
c.
d.
creating herbicide-resistant plants
producing DNA vaccines
gene therapy
synthesizing subunit vaccines
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$500 Question
If you have inserted a gene in the Ti plasmid,
the next step in genetic engineering is
a.
b.
c.
d.
inserting the Ti into Agrobacterium.
transformation of an animal cell.
transformation of E. coli with Ti.
splicing Ti into a plasmid.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Products of Genetic Engineering
$500 Answer
If you have inserted a gene in the Ti plasmid,
the next step in genetic engineering is
a.
b.
c.
d.
inserting the Ti into Agrobacterium.
transformation of an animal cell.
transformation of E. coli with Ti.
splicing Ti into a plasmid.
BACK TO GAME
© 2013 Pearson Education, Inc.
FINAL ROUND Question
One surprising finding in the Human Genome
Project was that “junk DNA,” which does NOT
code for a functional product, makes up what
percentage of the human genome?
a.
b.
c.
d.
90%
95%
98%
85%
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
FINAL ROUND Answer
One surprising finding in the Human Genome
Project was that “junk DNA,” which does NOT
code for a functional product, makes up what
percentage of the human genome?
a.
b.
c.
d.
90%
95%
98%
85%
BACK TO GAME
© 2013 Pearson Education, Inc.