Transcript All Alus are approximately 300 bp in length and derive

PV92 Insert Lab
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After presentation mark genotype of each
person that has a lane.
All Alus are approximately 300 bp in length and derive their name from a
single recognition site for the restriction enzyme AluI located near the
middle of the Alu element.
Human chromosomes contain about 1,000,000 Alu copies, which equal
10% of the total genome.
Alu elements probably arose from a gene that codes for RNA signal
peptide for export
Alu Insert
• Alu elements are found only in primates – the "monkey" branch of
the evolutionary tree, which includes humans. So, all of the
hundreds of thousands of Alu copies have accumulated in primates
since their separation from other vertebrate groups about 65 million
years ago.
• Once an Alu inserts at a chromosome locus, it can copy itself for
transposition, but there is no evidence that it is ever excised or lost
from a chromosome locus. So, each Alu insertion is stable through
evolutionary time. Each is the "fossil" of a unique transposition event
that occurred only once in primate evolution. Like genes, Alu
insertions are inherited in a Mendelian.
• An estimated 500-2,000 Alu elements are restricted to the human
genome. The vast majority of Alu insertions occur in non-coding
region. However, an Alu insertion in the NF-1 gene is responsible for
neurofibromatosis I.
Determine the Genotype of Each
Student ++ . +-, --
Genotypes of Students