Transcript 8.5 Translation

Wed., 10/26
Write Questions & ANSWERS! 
8.5 Translation
1. REPLICATE this DNA:
AGGTCATGC
2. TRANSCRIBE this DNA:
AGGTCATGC
3. Does DNA stay in the nucleus?
4. Does RNA stay in the nucleus?
8.5 Translation
8.5 Translation
So what did you learn yesterday???
replication
transcription
translation
8.5 Translation
8.5 Key Concept:
• Translation converts an mRNA message into
a polypeptide, or protein.
8.5 Translation
Protein “Synthesis”
• What does this MEAN?????
• Making proteins!
8.5 Translation
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•Let’s do this!!!
8.5 Translation
Amino acids are coded by mRNA base sequences.
• Translation converts mRNA messages into
•
polypeptides.
A codon is a sequence of three nucleotides
that codes for an amino acid.
codon for
methionine (Met)
codon for
leucine (Leu)
8.5 Translation
• The genetic code matches EACH codon to its amino acid or function.
The genetic code matches each RNA codon with its amino acid or function.
– three stop
codons
– one start
codon,
codes for
methionine
What amino acid is coded by GGA?........UGG……ACU?
8.5 Translation
Set of 3
nucleotides, read
in order
Signals start of
translation; codes for
methionine
A 3-nucleotide
sequence;
CODES for an
amino acid!
Signals STOP of
translation
8.5 Translation
• A change in the order in which codons
are read changes the resulting protein.
• Regardless of the organism, codons code
for the same amino acid!!!!
8.5 Translation
• Ribosomes consist of two subunits.
– The large subunit has three binding sites for tRNA.
– The small subunit binds to mRNA.
•Site for translation
What is the function of mRNA?
•rRNA & proteins
•Catalyzes bonds between
amino acids
8.5 Translation
Amino acids are linked to become a protein.
• An anticodon is a set of 3 nucleotides that
is complementary to an mRNA codon.
• An anticodon is carried by a tRNA.
•Carries amino acids from
cytoplasm to ribosome
•1 end: anticodon
•1 end: amino acid
8.5 Translation
Set of 3 nucleotides,
read in order
Signals start of
translation; codes for
methionine
Site for translation
rRNA & proteins
Catalyzes bonds
between amino acids
A 3-nucleotide
sequence;
CODES for an
amino acid!
3 nucleotides on
tRNA, bind to
complementary
mRNA
Codons “code” for
same amino acids in
almost ALL
organisms!
Signals STOP of
translation
Carries amino acids from
cytoplasm to ribosome
1 end: anticodon
1 end: amino acid
8.5 Translation
Ribosome assembles @ start
codon
Complementary tRNA
anticodon pairs up to mRNA
codon in the ribosome
8.5 Translation
• For translation to begin, tRNA binds to a
start codon and signals the ribosome to
assemble.
– A complementary tRNA molecule binds to the
exposed codon, bringing its amino acid close
to the first amino acid.
What is the function of mRNA?
8.5 Translation
tRNAs keep bringing amino
acids to ribosome, which
moves down mRNA
Ribosome forms peptide
bonds b/w amino acids &
breaks bond w/its tRNA
8.5 Translation
– The ribosome helps form a polypeptide bond
between the amino acids.
– The ribosome pulls the mRNA strand the length
of one codon.
8.5 Translation
Keeeeeeeeeeps going til the
ribosome reaches the STOP
codon!
8.5 Translation
– The now empty tRNA molecule exits the ribosome.
– A complementary tRNA molecule binds to the
next exposed codon.
– Once the stop codon is reached, the ribosome
releases the protein and disassembles.
What is the function of ribosomes?
8.5 Translation