Transcript Influence of honey in mice bearing Ehrlich carcinoma

Influence of honey in mice bearing
Ehrlich carcinoma on immune status
Ahmed G. Hegazi; Eman H. Abdel- Rahman; Fayrouz
Abd Allah and Amr M. Abdou
National Research Center, Dokki, Giza, Egypt
ahmed@ahmedhegazi com
and [email protected]
www.ahmedhegazi.com
3rd International Conference and Exhibition
on Clinical & Cellular Immunology
September 29 - October 01, 2014 Baltimore,
USA
Influence of honey in mice
bearing Ehrlich carcinoma on
immune status
Introduction
•Cancer continues to represent the largest
cause of mortality in the world and claims
over 6 million lives every year (Abdullaev et
al., 2000).
•An extremely promising strategy for cancer
prevention today is chemoprevention.
• Plants, vegetables and herbs used in the folk and
traditional medicine have been accepted
currently as one of the main sources of cancer
chemoprevention drug discovery and
development (Abdullaev, 2001).
• Antioxidants play an important role in inhibiting
and scavenging radicals, thus providing
protection to humans against infection and
degenerative diseases (Hegazi & Abd El Hady,
2009 and Hegazi et al., 2014).
• There is a growing interest in the
pharmacological evaluation of various natural
products used in traditional medicine.
• Flavonoids, terpenoids, and steroids have
received considerable attention in recent years
due to their diverse pharmacological properties
including antioxidant and antitumor activity
(DeFeudis et al., 2003 and Takeoka & Dao, 2003).
• From this viewpoint the present study was
carried out to
• evaluate the antitumor activity,
• immunoglobulin levels,
• phagocytic activity
• lymphocyte transformations
• delayed hypersensitivity
• of coriander honey against Ehrlich ascites
carcinoma (EAC) in Swiss albino mice.
Coriander honey:
•Coriander honey was purchased from local
market in Egypt.
•Sterile distilled water was used to dilute
honey immediately before administration
by a stomach tube.
•Honey was used in a final concentration of
500 mg/kg/mouse.
Animals:
Studies were carried out using a total of 210
male Swiss albino mice weighting 22- 25 g
obtained from Animal House of National
Research Center, Giza, Egypt.
Ehrlich ascites carcinoma:
Ehrlich ascites carcinoma (EAC) cells were
obtained from Cancer Biology Section, National
Cancer Institute, Cairo, Egypt. The Ehrlich tumor
line was maintained, till the time of the
experiment in female Swiss albino mice by serial
intraperitoneal passage of 2×106 cells/mouse at
7-10 days intervals (Hegazi et al., 2014).
Standard anticancer drug:
5-Fluorouracil (Calbiochem, USA) was used as
a standard anticancer drug. 5-Fluorouracil
was injected intraperitoneally to mice at a
dose of 20 mg/kg body weight (Karthigayan
et al., 2007 and Balamurugan et al., 2010).
Experimental design:
•A total of 210 male Swiss albino
mice were divided into 6 groups
(n=35) with an average weight 2225 gm each as follows:
Groups
Group Name
Saline
1
Normal control
2
Coriander control
3
5-flurouracil control
4
EAC control
5
Coriander + EAC
6
5-flurouracil + EAC
+
+
-
Treatment
Coriander honey
EAC
+
+
-
+
+
+
5floururacil
+
+
• At the second day post inoculation with Ehrlich carcinoma 0.1 ml
of bovine serum albumin was inoculated intraperitoneally in each
mice in all groups as priming for the specific antigen used in the
skin test.
• Blood and serum samples were collected from 5 mice from each
group at 7, 14 and 21 days intervals post inoculation with Ehrlich
effusive carcinoma cells.
• Blood samples were subjected to determine lymphocyte
transformation as Hegazi et al. (1981) & (1985)
• phagocytic activity as Hegazi et al. (1995).
• While the sera sample were used for the detection of different
Immunoglobulin levels IgG, IgA and IgM (Deverill, 1978).
•After 14 days of the experiment 5 mice from
each group were subjected to 18 h fasting
before sacrificing.
•Anti- tumor effect of coriander honey was
assessed by
•observation of changes in body weight,
•ascetics volume,
•viable and nonviable tumor cell count.
• The remaining animals in each group were kept
• to check the mean survival time (MST) of the tumor
bearing hosts
• and increase in life span percentage (ILS %).
• MST of each group containing five mice were
monitored by recording the mortality daily for 7
weeks and ILS % was calculated using the following
equation: MST= (Day of first death + Day of last
death)/2. ILS % = [(Mean survival time of treated
group/mean survival time of control group) -1] X 100
(Mazumder et al., 1997 and Gupta et al., 2000).
• Blood and serum samples were collected from 5 mice
from each group at 7, 14 and 21 days intervals post
inoculation with Ehrlich carcinoma cells.
• Blood samples were subjected to determine
• blood lymphocytic count,
• lymphocyte transformation
• The phagocytic activity
• While the sera sample were used for detection of
different Immunoglobulin levels by quantitative
determination of IgG, IgA and IgM (Deverill, 1978).
• At 72 hours before the end of the experiment
a 0.1-ml of bovine serum albumin was
inoculated in the left footpad of the mice
where the right foot pad was inoculated with
saline.
• Skin thickness was measured, 24 hours and
72 hours post inoculation to evaluate the
delayed type hypersensitivity as Cheville and
Beard (1972); Hegazi (1981) and Hegazi et al.
(1985).
Influence of honey in mice bearing Ehrlich
carcinoma on immune status
Influence of honey in mice bearing Ehrlich
carcinoma on immune status
Influence of honey in mice bearing Ehrlich
carcinoma on immune status
Influence of honey in mice bearing Ehrlich
carcinoma on immune status
Influence of honey in mice bearing Ehrlich
carcinoma on immune status
Influence of honey in mice bearing Ehrlich
carcinoma on immune status
Effect of coriander honey on hematological parameters in EAC
bearing mice.
Effect of coriander honey on IgM level in mice- bearing Ehrlich
carcinoma:
Effect of coriander honey on IgG level in mice- bearing Ehrlich
carcinoma:
Effect of coriander honey on IgA level in mice- bearing
Ehrlich carcinoma:
Effect of coriander honey on phagocytic activity level in micebearing Ehrlich carcinoma:
Effect of coriander honey on Lymphocyte transformation (stimulation
index using PHA) level in mice- bearing Ehrlich carcinoma:
Effect of coriander honey on Delayed hypersensitivity (skin
index / mm thickness): in mice- bearing Ehrlich carcinoma:
Influence of honey in mice bearing
Ehrlich carcinoma on immune status
Ahmed Hegazi 
National Research Center Dokki, Giza, Egypt 
Prof. Dr. Ahmed Hegazi •
Professor of Microbiology and Immunology •
National Research Center, Dokki, Giza, Egypt
President of Egyptian Environmental Society for
uses and production of bee products
Secretary of Egyptian Society of Apitherapy
Secretary General of African Federation of
Apiculture Associations
Member of Apitherapy Commission ,
APIMONDIA
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