Transcript XXCEL Strategic Communications Presentation

ELISA Testing
What is ELISA Technology?
• Enzyme-Linked Immunosorbant Assay
ELISA Testing
What is the between Antigen and Antibody testing?
Which does an ELISA test use?
BOTH
The Assay
• ELISA assays use antibodies that are covalently
linked ("conjugated") to enzymes. Antigen is bound to
a plastic well, and enzyme-linked antibody is bound
to the antigen. Unbound antibody is rinsed away. The
amount of enzyme remaining, and hence the amount
of antibody bound, is reported by adding a substrate
that changes color when acted upon by the enzyme.
The absorbance of the resulting color is proportional
to the amount of enzyme bound to the well, hence to
the amount of antibody, hence to the amount of
antigen. Thus, color reports antigen,
quantitatively.
ELISA Testing
• Well Tests – Commercial laboratory
settings.
– Handle large amount of samples
– Lower cost
– Labor intensive
ELISA Testing
• Well Testing
– Heartworm Wells (IDEXX, Synbiotic)
– Feline Leukemia (Labs)
– HIV
– Immunology
– Seed Purity
ELISA Testing
• Rapid Assays – In-clinic or patient side
testing
– Immediate results
– Fewer steps
– Higher cost
ELISA Testing
• Rapid Assays
– Heartworm
– FeLV/FIV
– Parvo
– Pregnancy
IDEXX ELISA Tests
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Snap®
Well
Heartworm
Lyme/ E.canis
Parvo
FeLV/ FIV
Giardia
Foal IgG
Feline Heartworm
Bovine Spongiform Encephalopathy
Chronic Wasting Disease
Classic Swine Fever Virus
Avian Influenza
Avian Mycoplasma
Equine Infectious Anemia
Lyme C-6 Antibody
IDEXX Snap® Device
How it works….
IDEXX Snap® Device
How it works….
This is why it is
called “Snap”
IDEXX Snap® Device
How it works….
IDEXX Snap® Device
How it works….
IDEXX Snap® Device
• Things to remember when running an
IDEXX Snap® Test.
– Know how many drops of blood and how
many drops of conjugate.
– 4 Whole blood, serum, or plasma
– 3 Drops conjugate
Standardized for all Snap Tests
IDEXX Snap® Device
• Things to remember when running an
IDEXX Snap® Test.
– “Snap” the device
– Does the test kit require refrigeration?
Questions?