Principles and Practices of Biosafety
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Transcript Principles and Practices of Biosafety
Human and Other Primate
Cells and Tissues
Human Source Material
Blood and blood products
Vaginal secretions
Semen
Amniotic fluid
Unfixed tissues
Cerebrospinal, synovial,
pleural, pericardial and
peritoneal fluids
Cell cultures
Saliva
Urine
Tears
Sputum
Feces
Vomit
Other excretions and
secretions
Second column not covered in Bloodborne Pathogen Standard, possibly not
occupationally related.
Human Source Material
May transmit infectious agents
Imperfect knowledge of infectious status
Incubation period (asymptomatic)
No test for every pathogen
Most tissues and body fluids
Bloodborne Pathogens (HBV, HCV, HIV,
HTLV-1)
Pathogens causing Malaria, Syphilis,
Babesiosis, Brucellosis, Leptospirosis,
Arboviral infections, Relapsing fever,
Creutzfeldt-Jacob disease and viral
hemorrhagic fever
Cell Culture Risks
Contaminating pathogenic agents
natural (often zoonotic) or inadvertent
~20 LAIs from primary cultures in last 30 years
e.g., Herpes B (CHV-1), prions
Oncogenic potential
could be oncogene or oncogenic agent
e.g., HPV-18, MPMV genomes in HeLa cells
Unexpected (adventitious) agents
e.g., SIV, STLV, SV5 in primate cells, HHV-8
in BCBL-1 cells
Hazardous chemicals added to culture medium
Cell Culture under Bloodborne
Pathogen Standard
ABSA requested OSHA’s interpretation in 1994:
Do human cell cultures fall under the
Bloodborne Pathogen (BBP) Standard?
Response:
All primary human cell cultures (explants) and
subsequent in vitro passages fall under the
BBP Standard
To be exempted from the BBP requirements,
cell strains and lines must undergo testing and
characterization (documented) for bloodborne
pathogens (not just HBV, HCV and HIV)
Cell Culture Safety
Extend Universal/Standard Precautions to all
human and animal cell cultures
Consider working at BSL 2 (most work
there already to protect the cell cultures)
Handle all cultures in a biosafety cabinet
If human origin and not demonstrated to
be free of human bloodborne pathogens,
adhere to requirements of the BBP Standard
Wear PPE appropriate to human source material
Summary
Human Source Materials
May be regulated
Can be biohazardous
Use Universal Precautions at
all times
Visible blood means
increased risk
Don’t consider “normal”
source
Human and Nonhuman Primate Cell
Cultures
Treat human cultures as
possible biohazards
Beware of non-human
primate cells
Beware of CNS, corneal,
pituitary cells
Some cells may be OK at
BSL 1