Transcript Lab. Safety
Lab. Safety, Microscopes and Colony Morphology Supplemental instruction Fall 2011 Chattahoochee Technical College Picture from http://www.toonpool.com/cartoons/MICROSCOPE%20LOOKING_27378 http://infectionnet.org/supporting/candida-plate/ Designed by Pyeongsug Kim ©2010 [email protected] Lab. Safety Food and Drink Not allowed. Safety goggles, Please…. Dispose materials in proper place. Picture from http://nobel.scas.bcit.ca/debeck_pt/science/safety.htm www.google.com/images Designed by Pyeongsug Kim, ©2010 www.science-i.com Dispose materials Bacteria containing solid materials. To biohazard can. Microscopic slides To glass disposal box Bacteria containing liquid test tubes. To waste rack Picture from http://electronmicroscopy.org/Histology_Disposal.htm http://www.globescientific.com/20-21mm-40place-plastic-test-tube-rack-p-1045.html Designed by Pyeongsug Kim, ©2010 www.science-i.com Dispose agar plates In order to dispose agar plate, wrap the plate with lab. film before putting the biohazard can. Picture from http://www.2spi.com/catalog/supp/parafilm.php https://mycotopia.net/forums/agar-strain-isolation/43307-agar-plates.html Designed by Pyeongsug Kim, ©2010 www.science-i.com What type of microscope are you seeing? Light compound microscope In which object do you need immersion oil to see the bacterial sample? 100X objective Why do you need oil-immersion? (how the oil-immersion work?) Bacteria is too small; Oil – to increase the magnification and to remove light refraction and to improve resolution. Designed by Pyeongsug Kim, ©2010 www.science-i.com What are the names of these parts? Write each function. A The answer is in your textbook or lab. notebook. B E C D Designed by Pyeongsug Kim, ©2010 www.science-i.com 40 X 10 X 100X Total magnification on the slide in view? 100x 10 X Total magnification on the slide in view? 400x 100X 40 X You want to see in 600 times magnification. Which part do you change to? (turn into..) 60x objective Designed by Pyeongsug Kim, ©2010 www.science-i.com Colony Morphology Picture from http://inst.bact.wisc.edu/inst/index.php?module=book&func=displayarticle&art_id=119 Designed by Pyeongsug Kim, ©2010 www.science-i.com Colony Morphology and Arrangements -Cocci •Diplococcus: cocci arranged in pairs. •Streptococcus: cocci arranged in chains •Staphylococcus: cocci arranged in irregular, often grape-like clusters Diplococcus Staphylococcus Streptococcus Picture from http://faculty.ccbcmd.edu/courses/bio141/lecguide/unit1/shape/diplo.html http://www.flashcardmachine.com/microbiology-lab-quizncc.htm Designed by Pyeongsug Kim, ©2010 www.science-i.com Colony Morphology and Arrangements -Bacillus(Rods) • Bacillus: a single bacillus • Diplobacillus: Bacilli that remain in pairs after they divide. • Streptobacillus: bacilli in chains • Coccobacillus: oval and similar to a coccus Bacilli Streptobacilli Picture from http://faculty.ccbcmd.edu/courses/bio141/lecguide/unit1/shape/diplo.html http://faculty.ccbcmd.edu/courses/bio141/labmanua/lab1/lab1.html Designed by Pyeongsug Kim, ©2010 www.science-i.com Picture from http://classes.midlandstech.edu/carterp/courses/bio225/chap04/lecture2.htm Designed by Pyeongsug Kim, ©2010 www.science-i.com