Transcript Origin and Evolution of Viral GPCR

Presentation
Bio Control Element
BY Qing Ma, Qian Cong, Shao wenjun
Mar 2, 2008
Outline
•Invention in iGEM should be biobrick with broadly usage
Original
Thought
•How to realize feedback (positive and negative)
•Use what signal: temperature, pH, [O2], concentration…
•Apply cold- and hot- sensitive promoter in E. coli
Current
Proposal
Potential
Utility
•Apply [O2] sensitive promoter in Cyanobacteria
•Download regulation or denotation—pharmacy use
•If the feedback is realized, this proposal could be used
in various circuit
•Anything relates to temperature mobility
2
Potential Usage for Temperature-Sensitive-Promoter
Based Element
•Pharmacy
--To sense temperature changing of affected person
--To control time and dosage of drug
•Environment
--To sense temperature related environment changing
--To promoter corresponding reaction
•Cell Factory
--To sense temperature changing in microcosmic
--Enable the microbe to regulate their efficiency automatically
3
Bacteria and human diseases
----guard and arrow
Type1:
Guard
• 形式：单个游离细菌体（温控释放型）
• 作用对象: 引起体温变化的病毒性疾病
（SARS， 禽流感，普通流感）
• 作用：释放小量药物或特异性标记病毒
Controlled by bidirectional promoters
----survive vs. self-bomb
Type 2 Arrow
• 作用对象：肿瘤型癌症
• 形式：脂质体型包裹（氧控释放型）
• 作用：定位释放较大量的抑癌药物
Problems
• 细菌本身的抗原性
• 药物包裹
Thank you
Any advice is welcome!