Transcript Singapour_presentation_iGEM2

IGEM TEAM
EPF-LAUSANNE
WHAT IS IGEM?
International Genetically Engineered Machine
Having received a kit of biological parts from the iGEM
Registry, we start to build our biological systems over the
summer.
The perspective of the Jamboree in November at MIT and
the project design is exceptionally motivating, in addition
to the fact that this is a good learning experience.
OUR TEAM & OUR SCHOOL
THE PROJECT: WET LAB PART
THE PROJECT: WET LAB PART

Cloning Parts:
 using the BioBrick
protocol of Ginkgo
Bioworks
THE PROJECT: MAKING A PART
THE PROJECT: WET LAB PART

The BphP / PpsR system
THE PROJECT: MODELING PART-AIM
Goal: Enhance the binary effect of the light switch
Molecular dynamics (MD) simulation of the photosensitive
protein domain on computer for two protein states:
-The light activated state
-The ground state (protein without light excitation)
Study of both state stability (statistical analysis)
Determination of amino acids that could be mutated to enhance
stability of light activated state
Mutation of the amino acids and test the switch in vivo
THE PROJECT: MODELING PARTPRELIMINARY RESULTS

First nanosecond MD simulation:
THE PROJECT: APPLICATIONS

Merck-Serono interests:

using light to induce gene transcription in mammalian
cells
Our Mentors:

Sebastian Maerkl Laboratory of Biological Network

Bart Deplancke Laboratory of Systems Biology and Genetics

Matteo Dal Peraro Laboratory for Biomolecular Modeling

Felix Naef Computational systems biology
Characterization (STI/SV)
THANK YOU!