Transcript Designing DNA Nanostructures to encapsulate and

Protein delivery:
DNA nanostructures and cell-surface targeting
Harvard iGEM
August 27, 2006
The Machine
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Goal: Future modularized drug delivery
target cell
DNA Nanostructures Overview
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Can design DNA double helices to stick
together and form interesting structures.
 Dr. Ned Seeman, NYU
 Dr. William Shih, Harvard
 Paul Rothemund, Caltech
A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron
WILLIAM M. SHIH, JOEL D. QUISPE & GERALD F. JOYCE Nature 427, 618ﾐ621 (2004); doi:10.1038/nature02307
Motivation: Why DNA?
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Fascinating area of research
The power of DNA
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Watson-Crick base pairing is enormously
strong
Self-assembly
Highly programmable, designable
Specificity - targeting to cells
Design Details
Design Details: Scaffolded Oragami
Design Details: Scaffolded Oragami
Design Details: Scaffolded Oragami
Design Details: Positional Control
Design Details: Positional Control
Design Details: Positional Control
Design Details: Positional Control
Design Details: Positional Control
Design Details: Positional Control
Progress
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Built a number of barrel designs
Exciting EM Images
Purifying Nanostructures (nearly there
after 1 month of trials)
Exciting EM Images
Exciting EM Images
EM Images (snakes on a grid)
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Images courtesy Shawn Douglas
c5.0 barrel
(10 nM), 0.7%
uranyl
formate
Appear to be
lining up end
to end,
probably
because of
the stain
To be continued
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Can a protein be
protected from
protease if
attached inside the
box?
Lid attachment
Lid removal
protein
protease
protease
protein
Acknowledgements
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Harvard TFs - Shawn Douglas, Nick Stroustrup,
Chris Doucette
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Harvard advisers - Dr. William Shih, Dr.
George Church, Dr. Pamela Silver, Dr. Alain Viel,
Jagesh Shah, Dr. Radhika Nagpal
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iGEM ambassadors
iGEM directors
Dr.