Transcript iGEM Fusion

Science, Technology, Engineering, and
Math all rolled up into iGEM
Ung.igem.org/High_School_Division
Synthetic Biology
Foundation Science for iGEM
Synthetic Biology take molecular genetics and apply engineering principles:
Standardization:
Development of systems that are standards in the biological world
ex. Restriction enzyme systems, promoters/translational unit
systems
Decoupling:
Separating a complex system into simple systems that work
together
ex. Building a fragrance free chassis, building tolerance to salt water,
sense arsenic, and fragrance system. When they are put together
they can make a bacteria that can tolerate salt water, sense arsenic
and release a fragrance in the presence of arsenic.
Abstraction:
Systems can be thought of in a hierarchy in two forms
Work in one level and disregard the other levels
Allow for the exchange between levels
ex. Relationship between RBS and ORF
iGEM: International Genetically Engineered Machine
Teams design, engineer, and build a biological machine from standard parts
Team structure:
All students should have beginning biology
Select students that are willing to work after school
May want students that have a computer background
Timeline
Flexible schedule dependent on the students
Afterschool activity
Correlate with curriculum in a class
Registration is from Mid August to Mid November
register at http://ung.igem.org/Main_Page
Team abstract are due in May with registration to the
jamboree. Declare a track for the jamboree. Teams will be
judged based on the track chosen.
Environmental, Health and Medicine, Informational
Processing, Food and energy, Foundational Advance,
Manufacturing, New Application, Software Tools
Jamboree is last Saturday in June
Budget
If you have the equipment, $300-$1,000 to do the lab work
Footsteps of an iGEMer: Fall Semester
Project idea:
Project brain storming session relevant to a need in society
Ethics of synthetic biology: Just because we can does not mean we do
Pick a Biological system
Research the host: based on the complexity and mentors available to your team
Make sure that you have competent cells to work with. Most cells that come from kits are considered
competent. You can look on the suppliers website to find out.
Research existing biological circuits
Team members research current parts that my be available for their system. These parts can be from
host or form the registry.
Design the new circuit to manipulate the host
Team members design the plasmid’s circuit to allow the host to become the machine. This can be a
multiple step system where students set goals to achieve during the building session. Students research
plasmid and part design to make their robot. They research current parts and systems to help with the
designing.
Community outreach program:
Team members talk about their project to the community .
Hook activities
smelly bacteria or photogenic bacteria
the
Footsteps of an iGEMer: Spring Semester
Building of the machine:
Teams build the machine using parts
Insertion into a plasmid
Transformation into the host cell
Standardization of results
Mathematically compute what the results mean to the user
ex. Level of florescence to concentration of promoter
Celebration of success
Jamboree
Teams get together to display their results at the poster session.
teams do a 20 minute presentation to judges.
Then
October
Teams can be volunteers for the Americas regional in Indianapolis
Teams present their posters during the poster session at the Americas Regional
Search for Parts
Parts from the Registry
Natural DNA
Acquire the Parts
iGEM parts that are received after registration
Extraction of the parts form the genome
Using primers ordered from a vendor
Write a lab that has the part and request that part
In the research of the part, students will read about labs
performing testing. Students can contact the lab and ask for a
sample.
3 A Assembly Method
Restriction Enzymes
Gibson Assembly
Primers and homologies
C:\Users\William Schini\Videos\The Gibson Assembly Song.mp4
Other avenues
Create new parts for submission into the
registry
Natural
Engineered
Create new plasmid backbone
Submit work for publishing
Community outreach:
2012 each team will be asked to develop
and implement a community outreach
program. Students can talk with local
groups about their project. Develop
activities and perform them with middle
school students.
Ethics of Synthetic Biology (GMO):
Team leaders can discuss the ethics of
GMO’s and understand the uses of GMO’s
Benefits for the student:
Any team competing in the jamboree will be able to present their
poster at the collegiate regional. Potentially to the final jamboree.
Students get to meet with leading scientists in the field of
molecular genetics, synthetic biology, biology, math, and engineering.
Connections developed through contact with mentors and
scientists.
Colleges do notice iGEM on applications, especially schools of
medicine.