Transcript LB145-lecture4

Announcements
• Contracts?
• Tailgate RSVPs due 14th.
• Reminder DRAFT1 due precisely at start
of lab both in print & on turnitin.com
• Thursday quiz or notebook check in lab
• Next week (ie week 4) start you 30 Days
experiment outside of lab and continue
PCR experiments in lab until succeed.
Outside/Prior to lecture
• Stand up if completed reading (10 pages)
Took Notes, Studied Notes
Inside/During lecture
• Learning how to use information and
knowledge in new situations
Outside/Prior to lecture
• Stand up if completed reading (10 pages)
Took Notes, Answered Qs, Studied Notes
Inside/During lecture
• Learning how to use information and
knowledge in new situations
What’s the story, the hypothesis and was it supported?
What’s the story, the hypothesis and was it supported?
What’s the story, the hypothesis and was it supported?
Prepare Paper “Structure” “Function”
Pre-test
Ribosomes
So what does a bacteria cell have & we don’t?
Plasmids
Cytoplasm
Chromosome
Flagellum
Plasma
membrane
Cell wall
So what does a bacteria cell have & we don’t?
A. The plasma membrane surrounding it
B. Chromosomes with DNA to make RNA
C. Ribosomes making proteins
D. The cell wall surrounding it
E. None of the above
Nucleus
Plasma membrane
Rough endoplasmic
reticulum
What does animal cell have that plants don’t?
Golgi apparatus
Chloroplast
Bacteria
Cell wall
What does animal cell have that plants don’t?
A. A single plasma membrane surrounding it
B. Mitochondria
C. A true Nucleus
D. The cell wall surrounding it
E. None of the above
Antibiotics that inhibit prokaryotic ribosomes
Ribosome
Antibiotics that inhibit prokaryotic ribosomes
A. Will stop bacterial protein synthesis
B. Will stop mitochondria protein synthesis
C. Will stop nucleus from making proteins
D. None of the above
E. More than one of the above
Collagen
Proteoglycan
complex
EXTRACELLULAR
FLUID
Fibronectin
Integrins
Plasma
membrane
Microfilaments
CYTOPLASM
Microvillus
Plasma membrane
Microfilaments (actin
filaments)
Intermediate filaments
0.25 µm
Euchromatin
Heterochromatin
Nucleolus
Nuclear envelope
Nucleus
1 µm
Nucleolus
Chromatin
Nuclear envelope:
Inner membrane
Outer membrane
Nuclear pore
Pore
complex
Surface of
nuclear envelope
Rough ER
Ribosome
1 µm
0.25 µm
Close-up of nuclear
envelope
Pore complexes (TEM)
Nuclear lamina (TEM)
0.2  m
Matrix
Cristae
Mitochondria
0.2  m
Intermembrane space
Outer
membrane
Free ribosomes
in the
mitochondrial
matrix
Inner
membrane
Cristae
Matrix
0.1 µm
Plasma membrane
Cytoplasm
Ancestral
prokaryote
DNA
Invagination
Endoplasmic reticulum
Nuclear envelope
Nucleus
Aerobic
heterotrophic
prokaryote
Endocytosis
Mitochondrion
(with extra mem)
How to test the endosymbiosis hypothesis…
What evidence could you look for?
(that might support or refute the hypothesis)
Design an experiment
to test the endosymbiosis hypothesis…
• Maybe using PCR
• Maybe using Microscopy
• Perhaps using streptomycin, erythromycin,
or tetracycline
• What’s the experiment, what is your
prediction based on the hypothesis?
Evidence for Endosymbiosis
Mitochondria/Chloroplasts
Bacteria
Morphology/Behavior: They swim around in your
cells, divide, look like cyanobacteria etc
Anatomy: Have 2 membranes, inner like bacteria
& outer like plasma membrane.
Molecular: Their own genes & ribosomes, that
function- sequences of genes, ribosomes, same
as bacteria, antibiotic, circular no histones
Pathogens: Undigested prey or parasites via endo
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