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PhD Position for the …
Biochemical and Molecular Analysis of
the Cell Wall Glyco(prote)ome
of Lactobacillus buchneri
Universität
für Bodenkultur Wien
Dept. of NanoBiotechnology
NanoGlycobiology
Group
Zentrum
für Nanobiotechnologie
NanoGlykobiologie-Gruppe
Time schedule: Start: February-April 2012; Duration: 3 years
Short summary of project
Lactobacillus buchneri strain CD034 possesses a complex cell wall
architecture. The main focus of the work lies particularly on the characterization of the glycosylated cell wall constituents of this organism,
such as the two-dimensionally crystallizing S-layer glycoprotein, a putative secondary cell wall polymer (SCWP) involved in S-layer anchoring,
and the identification of a predicted exopolysaccharide (EPS) which is
known from other lactic acid bacteria. In this context it should be mentioned that between S-layer carrying and S-layer deficient bacteria
strain-specific differences may exist regarding architecture, composition and function of specific cell wall constituents.
The presence of an S-layer glycoprotein on this GRAS organism, which
makes it an interesting candidate for possible future glycan-related
medical and/or biotechnological applications.
Research goals
 Bioinformatic analysis of the genome of L. buchneri CD034 for loci
Schematic presentation of the cell wall of
harboring genes involved in the biosynthesis of glycosylated cell
Lactobacillus strains with focus on glycowall components of L. buchneri CD034 (in collaboration with Prof.
sylated components
R. Grabherr, IAM, BOKU).
 Expression and purification of selected cell wall components from L. buchneri CD034.
 Structural and functional characterization of glycosylated cell wall components of L. buchneri CD034
(in collaboration with Prof. P. Kosma and Prof. F. Altmann, Chemie, BOKU).
Requirements
The candidate must hold a MSc degree and should have a sound background in molecular biology, microbiology, biochemistry. Practical experience in the field of glycobiology is of advantage but not mandatory. The
highly motivated candidate should be an excellent team player with the ability to work independently in an
interdisciplinary environment.
We offer
Motivating scientific environment at the Vienna Institute of Biotechnology, cutting edge research in
nanoglycobiology, weekly seminars, participation in workshops and international conferences. Stays in partner
laboratories abroad are possible.
Funding opportunity according to the guidelines of FWF (http://www.fwf.ac.at/de/projects/einzelprojekte.html).
Possibility of embedding the PhD thesis into the International PhD Programme BioToP (http://biotop.boku.ac.at/).
Applicants
Please e-mail your application with motivation, full CV and references along with contact addresses to:
Ao.Univ. Prof. Dr. Paul MESSNER
Department für NanoBiotechnologie, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Wien
e-mail: [email protected]; Phone: 47654-2202
University of Natural Resources and Applied Life Sciences, Vienna
Centre for Nanobiotechnology