Transcript Power Point presentation

Optimal Cutting Board
Disinfectants
By Joellie Hernandez
Introduction
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Individuals fail to clean cutting boards properly
and/or use damaged cutting boards.
If cutting boards are not cleaned well, harmful
bacteria can be found which can be ingested
through contamination with other foods.
Disease Control and Prevention Centers have
reported that about 73,000 individuals are affected
by E. Coli per year.
E. Coli currently accounts for two percent of all
cases of diarrhea in the western world.
Introduction Continued
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Animals such as cows, chickens, deer, sheep, and
pigs have been found to harbor E. Coli in their
intestines.
Contamination of meat occurs during the slaughtering
process through contact with feces and/or intestines
E. Coli infections causes abdominal cramps,
dehydration, bloody diarrhea and possibly seizures
and coma.
The ideal cutting board is a wood board that has a
smooth, hard surface
I will be testing both smooth and scuffed boards to see
what is the best disinfectant. I will try disinfecting with
Ajax, Dawn, Palmolive, Vinegar, Lemon and Water.
Hypothesis
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I believe that out of the
household cleaners, vinegar
will disinfect the cutting
boards the best because of
its antibacterial qualities; as
shown in a study done by Ph.
D. Patrick J. bird.
Knowing through my
research that scuffed boards
are bad to keep, I predict that
the smooth boards will give
the best results.
Methods
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I used a concentration of E.
Coli of ten parts water and one
part E. Coli
The solution was smeared
onto the cutting boards and
cleaned off with the
disinfectants one at a time.
Three trials of each solution
was made per board type.
The bacteria was kept at room
temperature and the colonies
were counted.
This is a picture of what E. Coli looks like
Growing E. Coli
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These are pictures of my experiment…
To the left is a
picture of one of my
agars after bacterial
colony growth.
To the left is
how I counted
the bacterial
colonies using
this grid.
Above are all my agars after
experimentation. (Some are
in the bags)
To the left is a
picture of one of
the cutting boards.
Above is how I kept my agars at
room temperature and free from
contamination or disturbance.
Results Page One
Household Substances
3500
Number of Bacterial Colonies
• Vinegar was the best
overall cleaner for both
smooth and scuffed
boards. Lime was the
second best cleaner
for the smooth boards
although it grew the
most bacteria on the
scuffed board.
Surprisingly, water did
not grow the most
colonies on the scuffed
board.
3000
2500
2000
smooth board
scuffed board
1500
1000
500
0
Water
Vinegar
Substance type
Lime
Results Page Two
Disinfectant Soaps
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Number of Bacterial Colonies
•From this graph, one
can see how much
more Palmolive grew
colonies than the
other soaps. From the
scuffed board,
Palmolive had almost
three times the
amount of bacterial
colonies than Dawn.
Ajax hardly grew any
colonies from the
smooth board and
had a low count for
bacterial colonies on
the scuffed board.
3000
2500
2000
smooth board
scuffed board
1500
1000
500
0
Dawn
Palmolive
Soap Type
Ajax
Conclusions
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When washing cutting boards, one should scrub off any food stuck
on them and wash them with hot, soapy water. Make sure you rinse
the boards well.
Wet or damp cutting boards should never be stored because these
are feeding grounds for possible bacteria.
Ajax was the best cleaner out of the household and
disinfectant soaps. Surprisingly, Palmolive grew the most
colonies out of all the substances. There is a big difference
between the graphs although Palmolive seems to have been
worse than water. That was least expected.
Future Studies
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For the future, this study can
give individuals better ideas
of how to take care of their
kitchen utensils.
It would be a remarkable
discovery to find a
disinfectant that is not
harmful to humans although
it would kill bacterium.
Scientists can go further and
see what chemical
compounds kill bacteria
most effectively.