Transcript 15.3 Gene Technologies in Detail

15.3 Gene Technologies in Detail
Mrs. Stipanovich
Tools to Manipulate Genes:
I.
Restriction enzymes= enzymes that
recognize a specific sequence of DNA
called a “restriction site.”
a. Used to cut DNA at these sites.
b. Creates “sticky ends” which have a
few bases on the end of it.
c. Sticky ends will easily bind to one
another.
Restriction Enzymes
Tools to Manipulate Genes
II. Gel Electrophoresis= The process by
which electrically charged DNA particles
suspended in gel move through the gel
because of an electric charge.
a. The DNA forms “lanes.”
b. Used to compare DNA, such as that of a
suspect or a victim.
Gel
Electrophoresis
Can you tell
who was the
killer at crime
scene 1?
Gel Electrophoresis in action!
Can I get a volunteer?
Tools to Manipulate Genes
III. Polymerase Chain Reaction (PCR)- A
process used to clone sections of DNA for
further study.
a. Imitates the natural process of DNA
Replication.
b. Called a chain reaction because it
repeats over and over (making many
clones).
PCR
•
Tools to Manipulate Genes
IV. Gene Recombination and Cloning
a. Can insert a gene into an organism that
replicates itself easily, such as bacterium.
Steps to Gene
Recombination &
Cloning
1.Cut the DNA samples.
2. Splice the sticky ends
together.
3. Place into host (bacteria).
4. Replicate gene (let the
bacteria breed).
5. Screen for the gene