Gene Therapy

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Transcript Gene Therapy

Connect!
 What is physical therapy?
 What is respiratory therapy?
 What does “therapy” mean?
 So, what do you think “gene therapy”
might mean?
4. Gene Therapy
 is the replacement of defective genes
with normal genes;
these normal genes must be transferred
into cells
 is being used to
treat cystic fibrosis
that affects the
respiratory system.
Viruses engineered
to carry the healthy
gene are sprayed
into the air
passageways of
CF patients.
The viruses carried
the healthy gene
into the patient’s
cells which become
healthy.
5. Separating DNA
 DNA fragments must be separated to be
analyzed in a procedure called gel
electrophoresis
 a mixture of DNA fragments is placed at one end
of a gel and an electric current is run through the
gel
 DNA molecules which are negatively charged
move toward the positive end of the gel; the
smaller the fragment of DNA the faster and
farther it moves
 Based on size and charge, the DNA fragments
make a pattern of bands on the gel that can
then be compared with other samples of DNA
 this technique can be used in paternity cases
or to determine guilt in criminal cases such as
rape and murder
Chunk
 What is gene therapy?
 Give an example of how it is used.
 What is the process of separating DNA
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fragments called?
Why is an electric current used in the gel?
Why do DNA fragments move towards the
positive end of the gel?
How are the fragments separated? (by what trait)
What is the end result?
What are some uses of electrophoresis?
 What do the bands in the
drawing of the agarose gel
represent?
 Which band(s) traveled
slowest?
Which band(s) traveled
fastest?
 Which is the positive end of the
gel?
 How many bands are shared in
common by all of the
individuals?
 Are there any bands which are
unique to only one individual?If
so, which one?
6. Karyotyping
 a chromosome sample can be taken from a person or
fetus for analysis
 pictures of chromosomes called karyotypes are arranged
in pairs and analyzed to diagnose diseases
 Analyze this karyotype.
and this…
and this…
JFF