Transcript biotechnology - Wikispaces.net

BIOTECHNOLOGY
DNA Fingerprinting
(Profiling)
Steps to DNA
Fingerprinting (Profiling)
 DNA
is extracted from sample
(skin, blood, etc.)
 DNA is “cut” with restriction
enzymes to make different
length pieces (restriction
fragment length
polymorphisms – rflps)
 fragments
are then
separated using gel
electrophoresis
 Electrophoresis Simulation
Website
http://gslc.genetics.utah.edu/units/bio
tech/gel/
 The
gel is stained or Xrays
taken, producing an
obvious band of
fragments resembling bar
codes on products
DNA fingerprints can be
used to...
 diagnose
genetic
disorders and identify
carriers
 analyze
evidence at crime
scenes (some placed in
CODIS-Combined DNA
Index System)
 evidence of paternity
 personal identity
Measure the Agar
Powder
Dissolve the Powder
and Heat
Casting the Tray
Loading the Wells
X-raying the Gel
Finished X-Ray of Gel
Complete the Gel
Electrophoresis Virtual Lab!

http://www.classzone.com/cz/bo
oks/bio_07/resources/htmls/virtu
al_labs/virtualLabs.html
Bacterial
Transformation
Bacteria will take in
foreign DNA and
manufacture
proteins coded for
in that DNA
Discovery led to
recombinant DNA
Frederick Griffith
Recombinant DNA (rDNA)
 DNA
from two organisms
are combined
 gene from one organism is
spliced into another one
Process
(1) Foreign gene is inserted into a
bacterial plasmid
(2) Bacteria take in the plasmid
(through transformation)
(3) Bacteria that are transformed are
cultured
(4) Bacteria produce the protein
Uses of Recombinant
DNA
 manufacture
of insulin
 manufacture of Factor VIII
 manufacture of HGH
(human growth hormone)
 TPA
for dissolving blood
clots (for patients that have
had heart attacks)
 vaccines such as for
hepatitis B and Gardasil (the
genes for virus proteins are
inserted into yeast)
 medication
that stimulates
growth of bone marrow after
a bone marrow transplant
 anti-cancer medications
 GM
(genetically modified)
food crops
In 2006, a total of 252 million
acres of transgenic crops
were planted in 22 countries
by 10.3 million farmers!
 most
GM food crops are
insect-resistant, droughtresistant, etc.
 some GM foods are
engineered to have
increased levels of vitamins,
such as golden rice
Are you eating GM foods?
More than 60% of food products
on grocery shelves contain
some crop that has been
genetically modified!
 For example, 81% of the
soybean products are GM
 60% of canola products are from
GM canola plants

Are you wearing GM plant
products?
 71%
of the cotton used in
textiles is GM
 (cottonseed oil can also be
found in salad dressing,
peanut butter, crackers,
cookies, and other foods)
 genetically
engineered
bacteria to clean oil spills
 larger amounts of ethanol
for fuel (biofuel)
 transgenic
animals in the
future could be used for
organ transplantation
Complete the Bacterial
Transformation Virtual Lab!!
 http://www.classzone.com/cz
/books/bio_07/resources/html
s/virtual_labs/virtualLabs.ht
ml
CLONING
 Organisms
with identical
DNA are clones
 Identical twins are clones
 Plants produced by “rooting”
are clones
 Some
animals are cloned
when they are damaged and
divided, such as earthworms
or starfish
 We have cloned some
animals by artificial means
Who Was Dolly?
In 1997, British scientists
successfully cloned a sheep by
transferring the nucleus from the cell
of an adult sheep into the cytoplasm
of an eunucleated fertilized egg. The
egg was then transplanted into the
uterus of a surrogate mother where
it developed like a normal zygote
into a lamb, which grew into a
normal adult sheep called 'Dolly'
Nuclear Transfer
Benefits of Cloning

Survival of endangered species
For example, a gaur
is an endangered
animal from Asia
and has been
successfully
cloned

Maintenance of high quality livestock for
food
The U.S. Food and Drug Administration, in
2008, concluded that “edible products
from normal, healthy clones or their
progeny do not appear to pose increased
food consumption risks relative to
comparable products from conventional
animals.”
importance –
cloning favorite pets is
expensive
 Economical
Cc (Carbon Copy)
 Genetics
Savings and
Clone was a company that
cloned pets for $50,000
 Without many customers,
the company closed
Statement issued 4/2/09 by the
BioTechnology Industry Organization
“The biotechnology industry has
developed and commercialized
more than 300 drugs and
diagnostics that are helping more
than 325 million people worldwide
and another 400 or so products are
in the pipeline.”
The Human Genome
Project
Project goals were to
 identify all the approximately
20,000-25,000 genes in human
DNA
 determine the sequences of the
3 billion chemical base pairs that
make up human DNA
 store
this information in
databases
 improve tools for data analysis
 we must address the ethical,
legal, and social issues that
may arise from the project
Francis Collins from
the publicly-funded
Human Genome
Project and J. Craig
Venter, head of the
private company Celera
Genomics, jointly
announce a working
draft of the human
genome
Gene Therapy
 Inserting
normal genes into a
person to correct a gene
mutation
 The “new” gene would be
transcribed, then translated
to produce normal protein
 Potential
cures for genetic
disorders such as
hemophilia, sickle cell, and
cystic fibrosis
 Or
for
cystic
fibrosis
In 1990, Ashanti
de Silva was the
first person to be
cured with gene
therapy; cured of
ADA deficiency (an
immune system
disorder)
STEM CELLS
Undifferentiated cells that have
the genes to become any type of
cell of that organism
 Primarily found in embryos, but
can also be found in adults,
such as stem cells in the bone
marrow that divide to make all
blood cells


If cells can be induced to
differentiate into a specific type
of cell, they can be used to
repair damage, such as people
with permanent spinal cord
injuries or potentially treat
Parkinson’s