Transcript IEA_flowchart_draft2

Genome
sequence
There is no existing
IEAGO annotation,
my dataset is unknown.
find
coding genes
Predicted
protein
coding genes
Find existing electronic
GO annotation
Bulk
Query
Read PMID:14681408 to learn how
these mappings are created.
Use InterProScan to find
Apply a GO mapping
domain matches, it will
to your dataset yourself
automatically
Download mapping files from
apply InterPro2GO
GO or EBI ftp sites
Assignments, if you do not
double check these then
they are an IEA assignment
Match nucleic acids or
protein Ids
in UniProt/MGI record
with the ids in your dataset
Record has Keyword
or Subcellular location
Swiss-Prot Keywords or
Subcellular Location
Attach UniProt record to the
marker that has
the nucleic acid id
Record has
domain
Attach domains to marker
Match Keywords or
Subcellular Locations
with downloaded GO
mapping tables
Download gene association
files from GO or
EBI ftp sites (GOA files/MGI/?)
Extract IEA from
Interpro2GO GO_REF:
Hamap2go GO_REF:
Spkw2GO GO_REF:
SPSC2GO GO_REF:
SPKW2GO GO _REF
Match InterPro domains
with downloaded
InterPro2GO mapping table
Attach GO term
to your record and
create reference
if submitting to GOC
If you need to convert your
identifier to another consider
downloading GOA Cross reference
(xref) files for human, mouse, rat,
chicken, Zebrafish,,Arabidopsis
and cow from GO or EBI ftp sites.
These include identifiers for Unigene,
Refseq NP and XP, TAIR, Ensembl,
H-Inv, UniParc,IPI, RGD, MGI,
TAIR, HGNC, ZFIN, EntrezGene
For other species not covered above
consider extracting IEA from NCBI
gene2go file or using database
identifier mapping service.(e.g xxx)
Record has Enzyme
Commission Number
Attach EC no. to Marker
MatchEC Number
with downloaded
EC2GO mapping table