Biology 6B Laboratory Report II

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Transcript Biology 6B Laboratory Report II

Biology 22
Laboratory Report 1
Spring 2010
Analysis of Body Color Inheritance
and Genetic Mapping in Fruit Flies
Refer to pages 1-231-26 of the
lab manual for a written
description of the lab report.
Format of Laboratory Report
Title
Statement of
Hypothesis
Results
Discussion
Conclusion
Title
The title should include
• the purpose(s) of the experiment(s)
• the name of the experimental organism or
system
eg. Saccharomyces cerevisiae or
cell lysate
Statement of Hypothesis
This section should include
the hypothesis you have formulated
for the inheritance of body color in
fruit flies.
a brief rationale for the chosen
hypothesis, considering the data as a
whole. You will give a more detailed
account of your reasoning in the
discussion section.
Results
• Present your experimental data in this section.
• Report your data and calculated values in tabular
format. Sample tables are shown on pages 1-25
1-26 of the lab manual. Place a meaningful
caption above each table. Each caption needs a
title and brief description explaining the source of
the data.
• Write a brief narrative to orient the reader to the
data shown in specific tables.
Results
Body Color
Inheritance
Phenotypes of Parents and
Offspring for each Cross
Proposed Genotypes and
Genetic Ratios for each Cross
Chi-square Analysis for each
Cross
Results
Three-Point Observed Numbers of Offspring in
Testcross
Each Phenotypic Class
Gene Order and Map Distances
between the genes
Discussion
Present your interpretation of the results in this
section.
Show how you deduced the hypothesis, explain
how it was tested and indicate whether the
hypothesis is supported by the data.
Provide a brief error analysis.
See pages 1-231-24 for questions to guide your analysis
of the data.
Discussing Body Color
Inheritance
Build your discussion around the answers to the
following questions:
 How did you determine whether the yellow body color
allele is dominant or recessive?
 How did you determine whether the yellow body color
allele is autosomal or sex-linked?
 Considering the chi-square analysis, how well does the
data fit your hypothesis?
 Propose plausible sources of variation for any
discrepancies you observe.
 Considering the entire set of data, has your hypothesis
been supported by this experiment?
Discussing Genetic Mapping
Build your discussion around the answers
to the following questions:
How did you determine the gene order?
How did you determine the gene distances?
How well does this data fit with expected values?
Propose plausible sources of variation for any
discrepancies you observe.
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Conclusion
This is a concise summary of all of the
results expressed in a 2-3 sentence
conclusion.
For example: “These data indicate that mutations in
genomic DNA increase with age and that the rate of
these mutations occurs faster in men than women.”
Length of each section
(written material)
Statement of Hypothesis: 1 paragraph
Results: 1-2 pages (longer if tables are included)
Discussion 2-3 pages
Summary: 2-3 sentences
TOTAL LENGTH: 4-6 pages of typed
material
Tables can be included on additional pages or
integrated into the text, as you prefer.