Transcript From Gene to Protein Part 2

FROM GENE TO PROTEIN
PART 2
Goal 1- Understand the process of transcription
• How is RNA made?
•How
to get from DNA to RNA
Goal 2- Demonstrate how RNA is made
• Transcription- Model activity
TRY THIS!

Molecular Genetics Alphabet
Online Activity
• Crack this code
DNA nucleotide triplet
ACG
Cys
RECAP: FROM GENE TO PROTEIN PART 1


What are the two main processes linking gene to
protein?
How RNA is made? (make a prediction)

What might the cell need/use to copy/transcribe DNA?
“Cookbook”
(genotype)
“Copy of recipe”
“Dish”
(phenotype)
TRANSCRIPTION: RNA SYNTHESIS
3
Phases:
Initiation
(start)
Elongation
(strand)
Termination
(stop)
To make a copy you need 3 things:
1.Something to copy – DNA! (cookbook)
2.Something to copy with – Nucleotides! (ingredients)
3.Something to make the copy – Enzymes! (recipe)
INITIATION (START)
Goal: to express (turn on)
Green fluorescent protein
DNA nucleotides
3’
5’
DNA template (the “something” to copy)
DNA nucleotides
3’
5’
1. DNA template (the something to copy)
2. RNA nucleotides
(the something to copy with)
3. RNA
polymerase
enzyme
(the
something to
make the
copy)
Transcription
factor
Green fluorescent gene
Promoter region
Termination sequence
5’
3’
Nucleotides
(T-T-A-T-T-T)
“TA-TA” box
Transcription
unit
“Downstream”
“Upstream”
Bind/unwind DNA
ELONGATION (STRAND)
Green fluorescent gene
3’
5’
ription
tor
3’
5’
Direction of RNA synthesis
5’
3’
Termination (stop)
Green fluorescent gene
3’
5’
5’
Transcription unit
Termination sequence
Transcribed (copied)
3
’
messenger RNA (mRNA)
Green fluorescent protein
Translated
TRANSCRIPTION VIDEOS

mRNA synthesis

Stages of Transcription