Transcript Translation (Protein Synthesis)

TRANSLATION
Protein Synthesis
Dr. Miranda YEOH
Kolej Matrikulasi Selangor
[email protected]
[email protected]
9/13/13
TUNE: GREEN GREEN GRASS OF HOME
At initiation stage,
mRNA binds to small ribosome sub-unit,
tRNA binds to start codon [AUG] on mRNA
Initiation complex is formed.
Large ribosome sub-unit comes and joins to the complex.
Aminoacyl tRNA with complementary anticodon
Recognizes the next codon [on mRNA]; it enters A site
Peptide bond forms between first and second amino acids
While bond between tRNA in P site and methionine breaks
The first tRNA dissociates from the ribosome
It's free to fetch another amino acid.
Ribosome moves to next codon, tRNA at A translocates to P site,
More amino acids are added, polypeptide elongates,
Until the stop codon is reached at termination,
Stop codon binds with release factor.
This hydrolyses bond between
Polypeptide and tRNA in P site
Protein chain and mRNA leave the ribosome
And ribosome sub-units dissociate.
The old home town looks the
same
As I step down from the train
And there to meet me is my Mama
and Papa
Down the road I look
And there comes Mary
Her golden lips like cherry
It’s good to touch
The green green grass
of home.
At INITIATION stage,
mRNA binds to small
ribosome sub-unit,
tRNA binds to start codon
[AUG] on mRNA
Initiation complex is formed.
Large ribosome sub-unit
comes and
Joins to the complex.
9/13/13
The old home town looks the
same
As I step down from the train
And there to meet me is my Mama
and Papa
Down the road I look
And there comes Mary
Her golden lips like cherry
It’s good to touch
The green green grass of home.
The old home town looks the
same
As I step down from the train
And there to meet me is my Mama
and Papa
Down the road I look
And there comes Mary
Her golden lips like cherry
It’s good to touch
The green green grass of home.
Aminoacyl tRNA with
complementary anticodon
Recognizes the next codon
[on mRNA]; it enters A site
Peptide bond forms
between first and second
amino acids
While bond between tRNA
in P site and methionine
breaks
The first tRNA dissociates
from ribosome;
It's free to fetch another
amino acid
Ribosome moves to next codon,
tRNA from A translocates to P
site
More amino acids are added,
polypeptide ELONGATES
Until stop codon is reached at
TERMINATION
Stop codon binds with release
factor,
This hydrolyses bond between
Polypeptide and tRNA;
Protein chain and mRNA leave
the ribosome,
And ribosome sub-units
dissociate.
At INITIATION stage,
mRNA binds to small ribosome sub-unit,
tRNA binds to start codon [AUG] on mRNA
Initiation complex is formed.
Large ribosome sub-unit comes and
Joins to the complex.
Met
UAC
AUG
GCC
3’
5’
mRNA
Small ribosome subunit
9/13/13
ELONGATION
Ala
Met
UAC
CGG
AUG
GCC
Aminoacyl tRNA with complementary
anticodon
Recognizes the next codon [on mRNA]; it
enters A site
3’
5’
mRNA
9/13/13
P site
A site
Small ribosome subunit
Met
Peptide
bond
Ala
UAC
AUG
Peptide bond forms between first and
second amino acids
While bond between tRNA in P site and
methionine breaks
The first tRNA dissociates from ribosome;
It's free to fetch another amino acid
CGG
GCC
5’
9/13/13
P site
A site
Small ribosome subunit
3’
Ribosome moves to next codon, tRNA from
A translocates to P site
Met
Peptide
bond
Ala
lys
CGG
AUG
GCC
A A A
5’
9/13/13
P site
A site
Small ribosome subunit
A G U
CUU
3’
met
ala
More amino acids are added, polypeptide
ELONGATES
lys
ser
AUG
5’
9/13/13
GCC
A A A
leu
UGA
G AA
A G U
CUU
3’
P site
A site
Small ribosome subunit
met
ala
lys
ser
Until stop codon is reached at
TERMINATION
Stop codon binds with release factor,
This hydrolyses bond between
Polypeptide and tRNA;
leu
asp
his
leu
gly
Protein chain and mRNA leave the
ribosome,
And ribosome sub-units dissociate.
Some short peptide hormones may have only 9 amino acids
Stop codon
U G A
5’
9/13/13
3’
ACKNOWLEDGEMENTS
THANKS are due to the Director of Kolej Matrikulasi Selangor, Vice
Director, the Head of Science Department and all lecturers and staff.
You have all been most helpful and encouraging; and this has
facilitated my research efforts in Cooperative Learning, Multiple
Intelligences of Students, Independent Learning, and Innovations in
Teaching Biology.
9/13/13
Special thanks to…..
All lecturers of Biology Unit, KMS.
All friends in RnD
All friends in ICC (KIK)
All KMS staff: library, admin office and labs!
Most of all, I thank my family, friends and students
who have been my respondents, my photographers,
advisors in video editing, my friends and my best
critics. You have contributed great insights that
have enabled me to continue in lifelong learning and
to improve in my teaching and research.
9/13/13