Transcript Protein Synthesis Review Game File

DNA and Protein Synthesis
Review Game
ROUND 1 – 5 POINTS EACH
ROUND 2 – 10 POINTS EACH
ROUND 3 - 15 POINTS EACH
- Study Guide
- CRAM is under 12/5
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1. What are the three basic parts of a nucleotide
(RNA or DNA)?
1. What are the three basic parts of a nucleotide
(RNA or DNA)?
2. What would you expect to see at the
beginning of the first exon on the 5’ end of a
mature mRNA after the GTP molecule?
2. What would you expect to see at the
beginning of the first exon on the 5’ end of a
mature mRNA after the GTP molecule?
AUG – the start codon
3. What are the names of the eight individual
proteins within a nucleosome?
3. What are the names of the eight individual
proteins within a nucleosome?
4. What is it called when ONE gene can make
various proteins?
4. What is it called when ONE gene can make
various proteins?
Alternative Splicing
5. What is the job of the molecule below?
5. What is the job of the molecule below?
To bring amino acids
during translation.
(this is a tRNA)
6. During which process is mRNA created?
6. During which process is mRNA created?
Transcription
ROUND 2
1. What amino acid sequence will be made from
the following mRNA?
CCC UCA AAG GAA
1. What amino acid sequence will be made from
the following mRNA?
CCC UCA AAG GAA
Proline, Serine, Lysine, Glutamate
2. Match the following molecule with it’s role in
TRANSLATION.
A.DNA
1. Brings amino acid monomers
B.mRNA
2. Brings the coded instructions to
the ribosome
C.tRNA
3. Energy molecule used
D.GTP
4. Original template for code, not
directly involved.
E.rRNA
5. The site of protein synthesis
2. Match the following molecule with it’s role in
TRANSLATION.
A.DNA, 4
1. Brings amino acid monomers
B.mRNA, 2
2. Brings the coded instructions to
the ribosome
C.tRNA, 1
3. Energy molecule used
D.GTP, 3
4. Original template for code, not
directly involved.
E.rRNA, 5
5. The site of protein synthesis
3. During protein synthesis, the DNA triplet TAT is
going to be transcribed and translated.
What is the anti-codon that will eventually bind to
the mRNA codon transcribed from this DNA
triplet?
3. During protein synthesis, the DNA triplet TAT is
going to be transcribed and translated.
What is the anti-codon that will eventually bind to
the mRNA codon transcribed from this DNA
triplet?
UAU
4. Name each of the following parts of a DNA
molecule.
A.
C.
B.
4. Name each of the following parts of a DNA
molecule.
Hydrogen Bonds
Nucleotides
Nitrogen Base (purine)
5. Which amino acid will be attached to tRNA ‘I’?
5. Which amino acid will be attached to tRNA ‘I’?
Histidine
6. If the DNA strand is AAT GCT ACC GTA
What will the anticodon sequence be during
translation?
6. If the DNA strand is AAT GCT ACC GTA
What will the anticodon sequence be during
translation?
DNA strand:
AAT GCT ACC GTA
mRNA strand:
UUA CGA UGG CAU
tRNA sequence:
AAU GCU ACC GUA
If Adenine nucleotides makes up 12% of a
gene, approximately what % of the
nucleotides will contain Guanine?
If Adenine nucleotides makes up 12% of a
gene, approximately what % of the
nucleotides will contain Guanine?
38%
ROUND 3
1. How is it possible that a bacteria can produce
protein from an inserted human gene, exactly
how a human cell would?
1. How is it possible that a bacteria can produce
protein from an inserted human gene, exactly
how a human cell would?
We use the same genetic code.
2. If Beadle and Tatum hypothesis had been
correct, how many genes would be responsible
for the following pathway?
2. If Beadle and Tatum hypothesis had been
correct, how many genes would be responsible
for the following pathway?
4
There are four enzymes
One gene = One enzyme
3. When RNA Polymerase is building during
transcription, in which direction on the DNA
strand is it moving?
3. When RNA Polymerase is building during
transcription, in which direction on the DNA
strand is it moving? It does not matter how
3’ to 5’ on the DNA
the DNA is turned- it is
ALWAYS 3’-5’ on the
template strand.
4. If caps and tails were not added to mature
mRNA, why would that be a problem?
4. If caps and tails were not added to mature
mRNA, why would that be a problem?
They would get ‘cleaved’ or ‘cut up’ by enzymes in
your cells (that are there to help protect you from
viruses)
5. In the process below:
a. To what molecule is the spliceosome
attaching?
b. What is being cut out?
5. In the process below:
a. To what molecule is the spliceosome
attaching? Pre-mRNA
b. What is being cut out? introns
6. During translation, in what instance would a
mutation (an incorrect nitrogenous base in a codon) have no
effect on the protein being made?
6. During translation, in what instance would a
mutation have no effect on the protein being
made?
When the mutation
still codes for the
correct amino acid