無投影片標題

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Transcript 無投影片標題

Catabolism ( breaking down of molecules )
- usually releases energy
Metabolism
( all the chemical reactions
occur in a cell )
Aanabolism ( synthesis of molecules )
- usually requires energy
Amino acids may not be linked together
but are close distance to each other
Other amino acids
for maintaining
the correct
globular structure
E+S
ES complex
EP complex
E+P
Compare and contrast the “lock and key hypothesis” and
“induce fit hypothesis”
Differences
1. Initial shape of
active site
LKH
exactly fit with
substrate
2. Flexibility of
enzyme molecule
IFH
may not be exactly right
in shape to fit the
substrate
less
more
3. Change in shape
of active site
no
yes, induced by the binding of
substrate with active site
Similarities :
1. Similar reaction sequence : E+S ->ES complex ->EP complex ->E+P
2. Catalytic reaction occurs in active site of enzyme
Activator
(inorganic )
A
B
C
Factor :
pH
Temperature
enzyme Conc
Substrate Conc
Rate of enzymatic
reaction
Coenzyme Conc
.
Which is the limiting factor which
.
controls the rate of reaction ?
For fixed amount of substrate
Competitive inhibitor
Non competitive inhibitor