Transcript Biosynthesis of Amino Acids - Tennessee Wesleyan College

Biosynthesis of Amino Acids
Overview
Overview (cont)
Biosynthetic Families
Metabolic Precursors
a-ketoglutarate
Amino Acids
Glutamate
Glutamine
Proline
Arginine
3-Phophoglycerate
Serine
Glycine
Cysteine
Biosynthetic Families (cont)
Metabolic Precursors
Amino Acids
Oxaloacetate
Aspartate, Asparagine
Methionine, Threonine,
Lysine
Pyruvate
Alanine, Valine, Leucine
Isoleucine
Biosynthetic Families (cont)
Metabolic Precursors
Phosphoenolpyruvate
and erythrose 4-phosphate
Amino Acids
Tryptophan
Phenlyalanine
Tyrosine
Ribose 5-phophate
Histidine
a-Ketoglutarate Family
a-Ketoglutarate
Glutamate
Glutamine
Proline
Arginine
a-Ketoglutamate to
glutamate/glutamine
Glutamate + NH4+ + ATP
Glutamine synthetase
Glutamine + ADP + Pi + H+
a-Ketoglutamate + glutamine + NADPH + H+
Glutamate synthase
2 glutamate + NADP+
a-Ketoglutamate to
glutamate/glutamine
Net Reaction:
a-Ketoglutarate + NH4+ + NADPH + ATP
L-glutamate + NADP+ + ADP + Pi
a-Ketoglutarate Family
a-Ketoglutarate
Glutamate
Glutamine
Proline
Arginine
Synthesis of Proline
Synthesis of Proline
Synthesis of Arginine
Synthesis of Arginine
3-Phosphoglycerate Family
3-Phosphoglycerate
Serine
Glycine
Cysteine
Synthesis of Serine and Glycine
Synthesis of Cysteine
Oxoloacetate Family
Oxaloacetate
Aspartate
Asparagine
Methionine
Lysine
Threonine
Synthesis of Aspartate and
Asparagine
Oxaloacetate
Transamination from
glutamate
Aspartate
Amidation with
Glutamine donating
NH4+
Asparagine
Synthesis of Lysine, Methionine,
and Threonine
• See handout
Pyruvate Family
Pyruvate
Alanine
Valine
Leucine
Isoleucine
Synthesis of Alanine
Pyruvate
Transamination from
glutamate
Alanine
Synthesis of Isoleucine, Valine,and
Leucine
• See handout
Phosphoenolpyruvate and
erythrose 4-phosphate Family
Phosphoenolpyruvate
+
erythrose 4-phosphate
Chorismate
Tryptophan
Tyrosine
Phenylalanine
Synthesis of Chorismate
Synthesis of Tryptophan
Synthesis of Tyrosine and
Phenylalanine
Synthesis of Tyrosine and
Phenylalanine (cont)
Biosynthesis of Histidine
• See handout
Possible Test Questions
1) Provide the 6 metabolic precursors and
the amino acids which they lead to.
2) Duplicate the Amino Acid Synthesis
Overview Flow Chart. Expand the
sections need to show the synthesis of
Glutamate, Glutamine, Proline, Arginine,
Serine, Cysteine, Glycine, and starting
from Chorismate Tryptophan, Tyrosine
and Phenylalanine. (40 points!!)