Transcript Managing people in sport organisations: A strategic human

Expression of Eukaryotic Proteins
•A bacterial Promoter/terminator must be used
•Bacteria can’t process introns, so cDNA must be
used
Translational Expression Vector
•Insertion of your gene into the MCS must be done
just right to get the RBS at the optimum 8
bases away from start ATG
•Strong means that sometimes multiple termination
sequences are used
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FIGURE 10.3
Codon Usage Affects Rate of Translation. Bacteria prefer one codon for a particular amino acid to
other redundant codons. In this example, the ribosome is stalled because it is waiting for lysine tRNA
with a UUU anticodon.
Companion site for Biotechnology. by Clark
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http://bio.takara.co.jp/product/basic_info.asp?catcd=B1000420&subcatcd=B1000421&unitid=U100006426
Companion site for Biotechnology. by Clark
Copyright © 2009 by Academic Press. All rights reserved.
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Companion site for Biotechnology. by Clark
Copyright © 2009 by Academic Press. All rights reserved.
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FIGURE 10.15
Comparison of Recombinant Protein Expression SystemsEach protein expression system falls on a
continuum of worst to best for characteristics such as speed, cost, glycosylation, folding, and government
regulations. Transgenic animals (rabbit) and transgenic plants (plants) are discussed in <ce:intra-refstext>Chapters 14 and 15</ce:intra-refs-text><ce:intra-ref-end xlink:href="pii:B978-0-12-175552-2.000142"><ce:intra-ref-title>chapter 14</ce:intra-ref-title></ce:intra-ref-end><ce:intra-ref-end xlink:href="pii:B978-012-175552-2.00015-4"><ce:intra-ref-title>chapter 15</ce:intra-ref-title></ce:intra-ref-end><ce:intra-refs-link />.
The other symbols include mammalian cultured cells, insect cell culture, yeast, and bacteria.
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