Transcript Stable Isotope and Metabolomics Core Facility

Stable Isotope and
Metabolomics Core Facility
AECOM Diabetes Center
The Einstein Stable Isotope & Metabolomics Core is within the NIH funded
Diabetes Research Center at Einstein and anchors the CMCR Metabolomics Core.
The Core uses stable isotope flux and metabolite profiling to help formulate and
test hypotheses about the metabolic consequences of various changes in gene
expression and protein function, in order to guide further integrative systems
biology analyses of the underlying mechanisms in diabetes, insulin resistance,
obesity, and diabetic complications. The Core objectives includes assessments of
plasma, urine and tissue metabolite profiles for diagnosis/characterization of
physiological and pathophysiological states. These include key metabolites in the
glycolytic, gluconeogenic, pentose phosphate, and tricarboxylic cycle pathways,
fatty acid oxidation and complex lipids, such as ceramides, diacylgylcerols,
triacylglycerols (TAGs), and phospholipids. In the CMCR framework, the Core will
use these methodologies to explore the specific effects that radiation has on the
metabolic network of each organ, and to explore the metabolic consequences of
protein modification, by radiation, on the function of the metabolic network.
Albert Einstein College of Medicine
OF YESHIVA UNIVERSITY
CMCR-AECOM
Stable Isotope & Metabolomics Core
Location: Price 368
Director:
Irwin Kurland,
MD PhD
Lab Members:
Sandhya Narasimhan, PhD
Bhavapriya
Vaitheesvaran, PhD
Seahorse Extracellular Flux
Analyses
Metabolite Profiling
and Flux Analyses
Kirsten Hartil, PhD
Hui-Min Zhang PhD
LC/MS Specialist
Mitochondrial Physiology
Animal Research Coordinator
XF Applications in Cellular Bioenergetic Assays
Obesity/diabetes
XF96 Extracellular
Flux Analyzer
Translation to
Clinical Applications
Cell quality
Cardiovascular
research
Mitochondrial
Function
Fatty Acid Oxidation
& Glycolysis
In vitro Toxicity
Neurodegenerative
diseases
Cancer
Waters Aquity
High resolution
Chromatography
LC/MS Analysis
Simultaneous analysis
of multiple metabolites
Multivariate
Statistical
Analyses
Identification of unknown
LTQ-Orbitrap species by structure elucidation
Waters Xevo Tandem MS
Synapt G2 Linear Hybrid quadrapole
Time of Flight MS with MALDI
Synapt G2 itself
Synapt G2 with MALDI front end
GC/MS Analysis of Metabolites
Metabolites from the
glycolytic pathway,
TCA cycle, pentose
cycle and amino acids
Lipid profiling:
Fatty acids
DAGs, TAGs
Cholesterol
Stable isotope assessments
of dynamic glucose disposal
(SipGTT), lipogenesis ([2H]2O
or U-13C]lactate), glucose
production ([U-13C]glucose),
glycerol production/lipolysis
([2-13C]glycerol), Cori cycle ([U-13C]lactate)
Waters Premier GC-Time of Flight-MS