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Lecture 25 Web: pollev.com/ucibio Text: To: 37607 Type in: 169964 <your question> Drug therapy THF metabolism inhibitors would have strongest effect on: A. Stem cells B. Cancer cells C. All cells D. All dividing cells E. No cells Purines Uric acid Pyrimidines MeMalonyl semialdehyde Salvage pathways APRT GMP HGPRT IMP AMP The future Disease phenotype treatment Drug design & development Protein engineering Industrial applications Diagnostics Metabolic engineering Engineer a whole pathway? Large scale production? Biosynthesis: - Fuel - Nutraceuticals What else?!?!! Example of metabolic engineering Patnaik, R. and Liao, J. (1994). "Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield". Appl. Environ. Microbiol. 60(11):3903-3908 Example of metabolic engineering Patnaik, R. and Liao, J. (1994). "Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield". Appl. Environ. Microbiol. 60(11):3903-3908 Example of metabolic engineering Patnaik, R. and Liao, J. (1994). "Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield". Appl. Environ. Microbiol. 60(11):3903-3908 -Omics = Metabolomics! ENTIRE collection of metabolites! “Metabolome” Metabolome & drugs Kaddurah-Daouk, Rima, Bruce S. Kristal, and Richard M. Weinshilboum. "Metabolomics: a global biochemical approach to drug response and disease." Annu. Rev. Pharmacol. Toxicol. 48 (2008): 653-683. Discovering diagnostic markers Kenny, Louise C., et al. "Novel biomarkers for pre-eclampsia detected using metabolomics and machine learning." Metabolomics 1.3 (2005): 227-234. Differentiating disease conditions Kaddurah-Daouk, Rima, Bruce S. Kristal, and Richard M. Weinshilboum. "Metabolomics: a global biochemical approach to drug response and disease." Annu. Rev. Pharmacol. Toxicol. 48 (2008): 653-683. The Human Metabolome project Synthetic biology – “creating” life… “Build” a genome from scratch? What is “minimal” genome? …and beyond! Protein + metabolic engineering Any compound, any time! “Download” vaccine to personal biofactory?!