Transcript Folie 1 - Radboud Universiteit

Enzymes . Chemicals . Solutions .
NEWPROT
Kick-off meeting: 25.01.2012
Partner profile Enzymicals AG
Dr. Rainer Wardenga
www.enzymicals.com
Company Info
Start-up history
Spin off from the Institute of Biochemistry at the University of Greifswald
Founded in August 2009 as corporation
Management
Dr. Ulf Menyes (CEO)
Dr. Rainer Wardenga (CSO)
Supervisory Board
Prof. Uwe Bornscheuer (Chairman)
Dr. Dagmar Braun
Dr. Jürgen Eck
Ownership structure
Shareholders and strategic investor
Northern Germany,
Biotechnikum Greifswald
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www.enzymicals.com
Company Info
Start-up history
Spin off from the Institute of Biochemistry at the University of Greifswald
Founded in August 2009 as corporation
Management
Dr. Ulf Menyes (CEO)
Dr. Rainer Wardenga (CSO)
Supervisory Board
Prof. Uwe Bornscheuer (Chairman)
Dr. Dagmar Braun
Dr. Jürgen Eck
Ownership structure
Shareholders and strategic investor
Northern Germany,
Sinetific Campus Greifswald
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www.enzymicals.com
Company Info
Management and staff
Dr. U. Menyes (Chemist), CEO
Dr. R. Wardenga (Biologist and Biotechnologist), CSO
Dipl. S. Weber (Economist), finance and controlling
Company management
Biotechnologists (main focus molecular biology)
Biotechnologist (main focus biocatalysis)
Chemical technical assistant
Biological technical assistant
Scientific staff
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Company Info
Business Segments
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Partners
Institute of Biochemistry at the University of Greifswald (Prof. Bornscheuer)
B.R.A.I.N. AG
HERBRAND PharmaChemicals GmbH
Institute of Chemistry at the University of Rostock (Prof. Kragl)
Institute of Marine Biotechnology e.V. Greifswald
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Research & Development Along the Value Chain
Core competence in protein science and development of biocatalytic process
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Research & Development Along the Value Chain
Identification of suitable catalysts
Method development and routine application of HPLC, CE, GC and GC-MS
Design and application of spectrophotometric assays
Synthesis of screening substrates
Screening of enzymes or strain collections
Search in literature or pathway databases
Search of known or novel motives in sequence databases
Amplification of novel genes from genomic DNA
De-novo genome sequencing and analysis
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Research & Development Along the Value Chain
Cloning, expression and purification
Fully equipped molecular laboratories
State of the art expertise in molecular cloning
Huge repertoire of expression vectors
Well-founded knowledge of a range of recombinant expression systems
Approved solutions for proteins which are difficult to express
Screening of refolding conditions for aggregated proteins
Production capacity up to 30 L-scale
Catalysts are available in different grades of purity
Purification via affinity-chromatography or fusion-tags
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Research & Development Along the Value Chain
Cloning, expression and purification in collaboration with B.R.A.I.N AG
Production Systems:
Peptide and Protein Production in
 Escherichia coli / Bacillus sp.
 Pseudomonas stutzeri / Pichia pastoris
 Streptomyces sp.
 Aspergillus sp.
Optimized Production of Peptides or
Proteins in Industrial Scale
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Research & Development Along the Value Chain
Biochemical, physiochemical and structural characterization
Determination of catalytic properties and kinetic parameters
Characteristics in temperature- and pH-profiles
Investigation of storage and process stability
Properties via gel-electrophoresis and blotting
Secondary structure via CD-spectrophotometry
Screening for crystallization conditions via commercial kits
Access to MALDI-TOF, 2D-gel-electrophoresis
General immobilization studies
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Research & Development Along the Value Chain
Protein engineering
Selection of best engineering approaches for designated targets
Approaches often inspired by structural alignments or molecular modeling
Homology-model construction and hot-spot identification
Rational design of variants by site-directed mutagenesis
Directed evolution with high quality variant libraries
Selection assays and library screening with high-throughput systems
HT-screening of mutant-libraries
Access to 3-laser flow cytometer with cell sorter
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Research & Development Along the Value Chain
Application of best catalysts paired with optimized chemistry
Innovative synthetic routes using organic synthesis and biotransformation
Custom manufacturing of fine and special chemicals up to the multi-kg scale
Catalysis in multi-phase systems or ionic liquids
Membrane processes for catalyst recovery and downstream processing
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www.enzymicals.com
Research & Development Along the Value Chain
Application of best catalysts paired with optimized chemistry
Innovative synthetic routes using organic synthesis and biotransformation
Custom manufacturing of fine and special chemicals up to the multi-kg scale
Scope of our biocatalytic tollbox
Catalysis in multi-phase systems or ionic liquids
Membrane processes for catalyst recovery and downstream processing
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NEWPROT: Objectives
(R)-selective amine-transaminases
Catalyze the transfer an amino group from a primary amine to a prochiral ketone or aldehyde
Usable for the resolution of racemic amines or the synthesis of chiral amines
Pyridoxal-5‘-phosphate dependent
Typical amines : alanine, ethylamine, 1- and 2-propylamine, 1- and 2-butylamine ….
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NEWPROT: Objectives
(R)-selective amine-transaminases
Catalytic tool box for the evaluation of synthesis routes
Conversion of aliphatic, arylaliphatic and aromatic ketones with strict (R)-selectivity
pH-optima range between 7.0 and 8.5
Alanine serves as amine donor
Products obtained by asymmetric synthesis with ECS-ATA01-07.
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NEWPROT: Objectives
Baeyer-Villiger monooxygenases
Flavoenzymes from the class of oxidoreductases
Oxidation of linear, cyclic and aromatic ketones to esters or lactones
Usable with cofactor regeneration or as whole cell system
Known applications:
Biooxidation of functionalized cycloketones
Biooxidation of bicyclic ketones or chiral b-substituted cycloketones
Kinetic resolution of racemic a-substituted cycloketones
Regiodivergent biooxidation of fused bicyclobutanones
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NEWPROT: Objectives
Baeyer-Villiger monooxygenases
Catalytic tool box for the evaluation of synthesis routes
Baeyer-Villiger oxidation of aromatic and aliphatic ketones, able to perform sulfur oxidations
High regio- and enantioselectivity, NAD(P)H-dependent
O
O
O
O
O
O
Me
Me
ee > 98%
ee > 98%
O
S
O
S
ee > 99%
ee > 99%
Scope of the ECS biooxidation-toolbox.
O
O
O
Me
Me
O
Me
Me
ee > 99%
ee > 99%
O
O
O
O
ee > 98%
ee > 98%
O
O
O
O
HN
HN
O
O
O
O
ee > 99%
ee > 99%
Cl
Cl
H
H
H
H
ee > 99%
ee > 99%
O
O
O
O
Examples of products obtained from the corresponding
ketones with ECS-M01-07.
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Enzymicals AG
Walther-Rathenau-Straße 49a
17489 Greifswald
Fon: +49(0)3834 515-47-0
Fax: +49(0)3834 515-47-3
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www.enzymicals.com