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Misusing of steroids and anabolic hormones,steroid derivatives,are found world wide both intentional used and lack of knowledge. Prolong of steroids using may cause serious adverse reactions such as osteoporosis. Anabolic hormones misused in sport is mostly testosterone - Testosterone undecanoate - Testosterone propionate - Testosterone enanthate - Methyl testosterone - Objectives : to survey the using of anabolic hormones to study the relation factors to anabolic hormones using to analyse unknown samples by steroid test kits Population : Study in 143 fitness male from July to September, 2006 at a fitness sport club, Yala youth center, Thailand 1. The questionnaire was used to collect the data about - general information - fitness behaviors - food supplements - knowledge in fitness exercise and anabolic hormones. 2. Analyse unknown sample by steroid test kits used TLC (Thin Layer Chromatography) technique. 3. Descriptive statistics and Chi-square (2) were used for data analysis. Std.Prednisolone Std.Dexamethasone Std.Testosterone Blank Sample 1 Sample 2 Sample 3 Sample 4 Unknown analysis,4 samples in 22 persons,were not found steroidal contents. Fig 1 Thin layer chromatography of unknown sample analysis The study found that : 1. proteins,vitamins/minerals and herbal substances were used 25.4,22.9 and 18.1 percent consecutively. 2. There are anabolic hormones using 15.7 percent of those used food supplements (9.09 percent of the population). 3. The aims of anabolic hormones using are to improve body performance 42.2 percent and for body building 26.5 percent. 4. The objective of fitness exercise,the objective of food supplements using,the information retrieving, and the frequency of doing fitness significant relation to anabolic hormones using (p-value < 0.05). 5. Unknown analysis were not found steroidal contents. 1. There are anabolic hormones using 9.09 percent of population. 2. Relation factors to anabolic hormones using - the objective of exercise - food supplements - information retrieving - frequency of exercise those significant relation to anabolic hormones using in population (p-value < 0.05). 3. Unknown analysis were not found steroidal contents. The finding outcome of anabolic hormones using can be used to conduct learning activities or academic programs for population as well as for college or university students to improve their understanding and knowledge in fitness exercise and anabolic hormones. More over, as a pharmacist, we have to play an active role in consumer protection. Body performance and strong muscle will be improved without anabolic hormones but those : - enough quality food - more exercise Contact information Mr Awirut Singkun Pharmacy Department Sirindhorn College of Public Health,Yala Muang Yala 95000 THAILAND E-mail : [email protected] Mobile : (+ 66) 8 - 9617- 0007 Alan I. Leshner., Anabolic steroid abuse. National Institute on Drug Abuse, U.S. Department of Health and Human Services, National Institutes of Health, 2000. Deirdre Maclean and A. Shane. Testosterone and anabolic steroids. CATIE Factsheet, 1999. Glenn R. Cunningham and et al. Patient’s guide to low testosterone. The hormone foundation, 2003. H. Kern. Muscular and hypertrophy. Department of Physical Medicine and Rehabilitation Wilhelminen Spital, Austria, 2000. Jay R. Hoffman and Nicholas A. Ratamess. Medical issues associated with anabolic steroid use: Are they exaggerated Journal of Sports Science and Medicine., 2006; 5: 182-193. U.S. Department of Justice. Anabolic steroids : A dangerous and illegal way to seek athletic dominance and better appearance, Drug Enforcement Administration, Office of Diversion Control, 2004.