Transcript Environmental Engineering

Aerobic (Co)Metabolism of Chlorinated Ethenes by
Microorganisms that Grow on Organic Acids and Alcohols
Peter Bottomley, Daniel Arp, Mark Dolan, and Lewis Semprini,
Oregon State University
PCE
TCE
1,2-DCA
0
1,2-trans DCE
25
Induction of lacZ activity in
response to 50 μM CAH
3
2
PCE
0
TCE
1
1,2-DCA
• Conduct reactor tests where rates of substrate
addition can be controlled, including batch reactor
quasi-steady-state tests and chemostat tests, to
evaluate catabolite repression.
31%
27%
1,2-trans DCE
• Conduct biochemical and transformation studies with
Pseudomonas butanovora to characterize induction of
monooxygenases and provision of reductant for the
monooxygenase-catalyzed reaction.
45%
50
1,2-cis DCE
• Isolate cultures capable of direct metabolism of cisdichloroethene and vinyl chloride and characterize
their growth kinetics and substrate range.
55%
1,2-cis DCE
Research Objectives:
75
1,1-DCE
VC Epoxide
1,1-DCE
+
Buffer
NADH NAD
100
Buffer
H2O
H
Butanol
VC
O2
H
Butanol
H
Cl- + CO2 + H2O
Butane
H
C C
Cl CoM
Butane
C=C
H
Monooxygenase
Induction of BMO activity
(% of butane control)
Cl
Induction of BMO activity in
response to 50 μM CAH
Induction of lacZ
(fold increase over background)
H
O