Transcript Document

The world leader in serving science
Rhombix Solutions for Automated
Crystallization
2007 RAMC
Rhombix Product Line – supporting the crystallization workflow
Semi-automated
imaging
Rhombix
Software
Suite
MaX Workcell
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Automated imaging and storage
Instrument-Centric to Data-Centric Automation
Liquid handler –
Dispense screens into wells
Nanodispenser
Drop-making
Xtallization
Database
Experiment design and analysis
Imaging and Storage
Result: Isolated “islands” of automation and data stores -- No data connection
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New Product
New
MaX Workcell
Plate preparation
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MaX Workcell
 Features & Benefits
• All-inclusive product – pre-configured for quick
installation
• Seamless Workflow
• Tube-to-plate transfer
• Plate-to-plate transfer
• Custom screen building (gradients, additives,
etc)
• Nano Drop placement
• Sealing
• Multiple experiment design and submission
• Full set of SW tools incorporating experimental
data and automation control
• Small footprint
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Imaging Modes
Brightfield
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White LED light source
Eliminates heating of plates
Darkfield
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Changes the angle of incidence mitigating shadow regions
associated with Brightfield
Assists visualization of crystals
Polarization
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True polarization
Variable angle transmitter and analyzer
Exploits birefringent property of crystals
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Lighting and treatment options
Brightfield
 Darkfield
 Variable Angle Polarization
 Extinction
 Extinction + 45°
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 Extinction + 90°
Imaging Features
 Drop-finding
 X-focus (extended depth of focus)
 Others: autofocus, autoexposure, sharpening
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Rhombix Software Suite – Supporting the Crystallization
Workflow
Protein Crystallization Software Packages
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Reagent Manager
Screen Designer
Experiment Manager
Schedule Editor
Barcode Manager
Experiment
Design
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Deck Manager
Experiment Launcher
Screen Monitor
Vision Monitor
Equipment Monitor
Experiment
Execution
• Visual Query Tool
• Image Viewing and
Annotation (Clarix)
• Report Tool
Results Analysis
and Scoring
Optimization
Data Mining
• Screen Designer
• Visual Query Tool
• Image Viewing and
Annotation (Clarix)
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Knowledge
Rhombix Software
Fourteen Software Applications Built into an Cohesive Framework
Spanning: Reagent Management to Data-Mining
Userdefined
values
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Viewing and Annotation -- Clarix
 interface for viewing and annotating
images
 brings in information from database
(e.g. well contents & drop details)
 provides feedback to imaging
 link to Screen Design for optimization
screens
 link to data-mining
 link to scheduling
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Report Tool
 Report across users and
experiments
 Export to .xls, .pdf, HTML, xml
formats
 One means of data sharing
between sites (export at one
site, import at another site)
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Data Mining – Visual Query Tool
 Graphical data-mining SW for general
queries and experiment optimization
planning
 Links to Viewing application for display
of image results
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Screen Designer
 Graphical interface for
designing screens
 Allows concentration
and pH gradients
 Gradients across grids,
rows, columns
 Patterns of start/step,
start/end, center/step
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Import Utility
 Imports chemicals,
screening solution
formulations, screens,
and experiments
 Pair with report tool for
data sharing
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More Insight and Less Mystery in Crystallization
Tools for easy data retrieval and viewing
Tools for easy data entry
“Drop to Crystal” Workflow
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