Microbial Detection Arrays

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Transcript Microbial Detection Arrays

Microbial Detection Arrays
Dave Miller
Elizabeth Newton
Ted Schumacher
Shayla Stewart
Steven To
Charles Vaughan
Sameera Wijesinghe
Interim Review I
February 6th, 2007
Aerospace Senior Projects
University of Colorado – Boulder
Advisors: Dr. Forbes and Dr. Maslanik
Customers: BioServe and Tufts University
Briefing Overview
•
•
•
•
•
•
Overall Design Summary
Changes Since CDR
Test Plans
Schedule
Budget
Current Issues
2
Overall Design Summary
Autoclaves
Reagent Water Chamber
Peristaltic Pump Mounts
Top Shelf
Environmental
Chamber
Reaction
Chambers
(with Mixers)
Mixer Motor
Mounts
NOTE: Only
shows parts to
be manufactured3
Changes Since CDR
• Mechanical:
– Autoclave: Repositioning of screw holes and O-ring groove
– Mixer: Rearrangement of impellers, addition of bottom scoop
– Environmental chamber: Walls broken up to simplify
manufacturing and assembly
– Shelves and supports: Sizes standardized, COTS supports
• Electrical:
– Sensors: LM34 temperature sensors on autoclave, reagent
water chamber, and ambient
• PP-0079 thermistors on reaction chamber
• Additional thermistors placed on reaction chambers
• Software:
– Laptop hard drive for data storage
• Manufacturing:
– BioServe manufacturing parts of autoclave bodies (milling),
reaction chamber bodies, and mixers
4
Flat Scoop
1
TEC
AC
2
TEC
AC
1
TEC
RC
2
TEC
RC
2
Control
2
TEC
1
TEC
Autolcave
x
4
x
2
x
4
x
2
x
4
x
2
Autoclave
1
Control
x
RC
x
4
x
2
x
3
RC
1
Control
Mixer
2
heater
water
Reagent
H2
Autoclave
LED's
H1
Autoclave
Pump1
4
Output
Analog
x
x
output
Digital
Input
Analog
x
6
1
x
x
4
3
2
x
2
1
x
x
3
6
Board
Switch
2
2
x
2
x
2
x
2
x
2
Rectangular
Mixing Blades
1
x
3
x
2
2
Pump
Sensors
x
2
Computer
Distribution
Power
Supply
Power
Mixer
Mixer
2
Mixer
2
Control
Mixer
Changes Since CDR
Electrical System
Four Controlled
Items
5
Test Plan Flow Chart
Power Supply
Circuit
Reagent H2O
Containment
Switch Board
Circuit
Sample
Transport
Peristaltic Pump
Control Circuit
Mixer Control
Circuit
Autoclave
Heater
Software
Input/Output
Voltage
Integrated
Electrical
Subsystem
Mixing
Capability
Integrated
System
Verification
Sample
Sterility
Autoclave
Temp/Pressure
Autoclave
Kill Test
TEC Control
Circuit
Disassembly/
Reassembly
Rxn Chamber
Environ. Control
Electrical
Mechanical
Software
Biological
Integrated
6
Special Test Requirements
• Hazard Analyses
– Electrical: electrocution/fire
– Autoclave: pressure vessel (projectiles), hot
surfaces, sharp edges
– Assembly/Disassembly: sharp edges
– Environmental control: hot surfaces
– Integrated System: all of above
• Testing Locations
– BioServe Environmental Chamber
– BioServe Wet Chemistry Lab
7
Schedule
8
Budget
Expected
Actual
Electronics
$0
$0 (donated)
Sensors
$0
$0 (donated)
$1,328.53
$1,909.61
Computer/DAQ
$348.00
$606.00
Miscellaneous
$150.00
$328.01
$1,826.53
$2,843.62
Mechanical
Total
9
Current Issues
• Tubing:
– PharMed tubing does not come in 1” inner diameter
• Substitution has been found (Bioprene) but has not been
ordered
• TEC Controller:
– Power cord did not come with TEC controller
• Replacement can be fabricated or purchased
• Manufacturing:
– Two weeks behind schedule
• Offset by manufacturing contributions from BioServe
• Ordering:
– Pumps, thermostats, and TECs have not been
ordered
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Conclusions
• Manufacturing behind schedule
– Offset by BioServe’s assistance
• Most parts are ordered
– Tubing is only ordering concern
– Can be offset by finding other distributors
• Still on budget
– Over what was expected, but still under budget with
most parts ordered
• Mechanical, software, and electrical packages
approved
• Will be able to complete manufacturing and
testing by deadlines
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