Transcript Wisconsin Fast Plants - Hudson City School District

AP Lab 7
Wisconsin Fast Plants
“Who’s The Father?
What are Wisconsin Fast Plants?
Read the lab and do p.2
• Type of mustard, radish, cabbage plant
especially bred by Paul Williams of the
University of Wisconsin
WFP Life Cycle Video
• Fast Plants Time Lapse Video
See stems and ANL (purple) and
anl (non-purple or green)
Grow seeds in Petri dish
Use Lid as bottom
Other part as cover
Cut paper towel circle
• Wet paper towel well with
a pipette
NAME
Water is to break the seed coat
Germinates in a day or two
FRIDAY
-Mark Petri dish lid into two halves
-Label P on one side, F1 the other
-Moisten paper towel circle and place 5
seeds in each side of the lid
F2 WFP (1:57)
P
F1
Put you initials
on the Petri
dish cover
Root emerges with
root hairs to increase
surface area for
water absorption.
Petri dish at slight angle under a
light
It will sit all week-end so be sure there is
enough water in the Petri dish.
Day4 (MONDAY)
(cont. on next slide)
• Stem and leaves emerge
• Water F1 and P well.
• “Observing the Seeds” (make 3 quantitative
and 3 qualitative observations, sketch and
color, count the genotypes and phenotypes.)
• Attach a paper with these observations.
Monday: Now plant F2
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Place 10 seeds in a Petri dish
Two rows of 5
0
0
Water well.
0
0
Label F2
0
0
Cover
0
0
Label your initials
0
0
Put under light at
a slight angle
Tuesday
• Take data for F1 and P
• Check on seeds –add
water if needed
Day 7 (Friday)
• DATA COLLECTION DAY OF F2
Purple/Standard
Non-Purple/Standard
Purple/Rosette-Dwarf
Non-Purple/ Rosette Dwarf
(see pictures next slide)
Friday: Count F2
Purple/standard stem
Non-purple/Standard
stem
Rosette
dwarf
Collect Group Data
• P. 6
• Sketch plants, color
• Do dihybrid of the plant genotypes
Chi square Test
• Bozeman Biology Chi Square Test
• To test your null hypothesis
• Critical value and degrees of freedom
Day14-20 (flowers)
How do you grow WFP?
• Need constant light
How do you grow WFP?
• Seeds are tiny-do not
cover with very much
potting soil
How do you grow WFP?
• Need fertilizer
Label your quads
Green and Non-green leaves
• Wisconsin Fast Plants® F1 Non-Purple
Stem, Yellow-Green Leaf (F1
Anthocyaninless, Yellow-Green)
• F1 seed from cross of Non-Purple
Stem, Yellow-Green Leaf (anl/ygr) ×
Purple Stem (ANL/YGR);