Transcript Wisconsin Fast Plants

AP Lab 7
Wisconsin Fast Plants
“Who’s The Father?
What are Wisconsin Fast Plants?
FIRST: Read the lab and do p.2
• Type of mustard, radish, cabbage plant
especially bred by Paul Williams of the
University of Wisconsin
WFP Life Cycle Video
• WFP Time Lapse Video (scroll below life
cycle diagram)
See stems:PP or Pp (purple) and
pp (non-purple or green)
Standard (Tall) TT
Dwarf (tt)
Grow seeds in Petri dish
Use Lid as bottom
Other part as cover
Cut paper towel circles
• Wet paper towel well with
a pipette
NAME
FRIDAY –May 15 Work in Pairs
2 Petri dishes/table
-Mark Petri dish lid into two halves
-Label P on one side, F1 the other
-Moisten paper towel circle well and
place 5 seeds in each side of the lid
Put you initials
F2 WFP (1:57)
P
F1
on the Petri dish
cover
2 cm water
seeds above water line
tilt dish slightly
name on Petri dish
Petri dish at slight angle under a
light
It will sit all week-end so be sure there is
enough water in the Petri dish.
Water is to break the seed coat
Germinates in a day or two
Root emerges with
root hairs to increase
surface area for
water absorption.
Day4 (MONDAY)
(cont. on next slide)
• Stem and leaves emerge
• Water F1 and P well.
• “Observing the Seeds” (make 3 quantitative
and 3 qualitative observations, sketch and
color, count the genotypes and phenotypes.)
• Can also take a picture.
SOME TRAITS
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Non-purple (green) – recessive pp
Purple - dominant PP or Pp
Green – dominant pp
Tall – dominant T
Dwarf- recessive t
Clean out 1 Petri dish
Label F2
Save F1/P1 Petri Dish
Tuesday: Now plant F2
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Place 10 seeds in a Petri dish
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1 per table
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Two rows of 5
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Water well.
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Label F2
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Cover
Label your lab table and period
Put under light
0
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Day 7 (Friday) p. 6
• DATA COLLECTION DAY OF F2
Purple Tall
Green Tall
Purple Dwarf
Green Dwarf
(see pictures next slide)
Monday: ALL CLASS
TOTALS OF F2
Purple/standard stem
Non-purple/Standard
stem
Rosette
dwarf
Collect Group Data
• P. 6
• Sketch plants, color
• Do dihybrid of the plant genotypes
Chi square Test
• Bozeman Biology Chi Square Test
• To test your null hypothesis
• Critical value and degrees of freedom
Day14-20 (flowers)
How do you grow WFP?
• Need constant light
How do you grow WFP?
• Seeds are tiny-do not
cover with very much
potting soil
How do you grow WFP?
• Need fertilizer
Label your quads
Green and Non-green leaves
• Wisconsin Fast Plants® F1 Non-Purple
Stem, Yellow-Green Leaf (F1
Anthocyaninless, Yellow-Green)
• F1 seed from cross of Non-Purple
Stem, Yellow-Green Leaf (anl/ygr) ×
Purple Stem (ANL/YGR);