Transcript PPS

Adenovirus and Cryptosporidium coinfection in a Corn snake (Elaphe
guttata guttata)
Debabrata Mahapatra
Department of Infectious Diseases and Pathology
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
Case History (N08-13)
•
A 13 month old male corn snake was part of
the breeding stock of ~2500 mixed Colubrid
species.
Off-feed about 2 weeks and sharply declining.
Recently moved into a different facility.
•
Gross findings: No gross lesions.
•
•
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
Presented at SEVPAC 2008 – Permission
Section of stomach showing
mucosal hypertrophy and hyperplasia,
granted for use on SEVPAC website only
mononuclear infiltration in lamina propria and numerous cryptosporidia. (400x)
PV
PV
Sp
Sp
R
Presented
SEVPAC 2008 – Permissionvacuoles (PV) with sporozoites
EM of sporulating oocysts located
in atparasitophorous
granted for use on SEVPAC website only
(Sp) and residual body (R)
M
S
S
DB
FO
M
O
Presented at SEVPAC 2008 – Permission
EM of two schizonts (S) with mature
merozoites
(M). An oocyst (O) on the
grantedsickle-shaped
for use on SEVPAC website
only
left with feeder organelle (FO) that is separated from the host by a dense band (DB)
at SEVPAC 2008 – Permission
Section of intestine showing Presented
villous
blunting,
expansion
of lamina propria with
granted
for use
on SEVPAC website
only
mononuclear cell infiltration.
Presented at SEVPAC 2008 – Permission
Section of intestine showing prominent
largewebsite
basophilic
enterocyte intranuclear
granted for use on SEVPAC
only
inclusion bodies.
I
V
N
Presented at SEVPAC 2008 – Permission
granted(I)
for use
on SEVPAC
website onlyelectron dense viral particles (V)
EM of a intranuclear inclusion body
with
numerous
within a nucleus (N).
Morphological diagnosis
•
Proliferative gastritis, lymphoplasmacytic, diffuse,
moderate, with intralesional protozoal parasites
consistent with Cryptosporidium sp., stomach.
•
Enteritis, lymphoplasmacytic, multifocal, mild to
moderate, with enterocyte intranuclear inclusions
consistent with adenovirus, small intestine.
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
Cryptosporidiosis
• Cryptosporidium has been reported in over 57
different species of reptiles including:
• 40 species of snakes
• 15 species of lizards
• 2 species of tortoises
Of all reptiles, snakes are most severely affected.
Lesions are mostly restricted to stomach and intestine
with proliferative gastritis and enteritis.
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
Cryptosporidiosis
•
Lizards have a wider range of tissue tropism.
Identified in:
• In kidneys of Parson’s chameleon.
• In kidneys, salivary glands, aural and pharyngeal polyps in
green iguana.
Most common species affecting snakes is
Cryptosporidium serpentis.
• Recently, C. saurophilum has been described in
lizards.
•
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
Adenovirus
•
Adenoviruses are reported in:
•
12 different species of reptiles of the orders
Crocodilia and Squamata including
•
Serpentes (snakes) and Sauria (lizards) suborders.
•
Most of the cases are reported from zoological collection
and commercial breeders.
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
Adenovirus
•
Incriminated as the cause of gastroenteritis, hepatitis,
nephritis, pneumonia, and encephalitis.
•
Gastrointestinal lesions have been described in snakes
in the families Boidae, Colubridae and Viperidae.
•
Cytopathologic changes include:
•
•
•
•
Large eosinophilic to basophilic intranuclear inclusions
Nuclear swelling
Ballooning degeneration
Necrosis of organs
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
Conclusion
Primary infection with adenovirus might have
immunocompromised the snake and predisposed it to
secondary infection with cryptosporidia.
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
Acknowledgement
•
•
•
•
•
•
•
Dr. David Taylor
Dr. Lisa Farina
Dr. Mary Reinhard
Dr. Elliot Jacobson’s lab
Lou Ann Miller
UF CVM Histopathology lab members
Residents
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
???
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only