Transcript Document

Myelodysplastic Syndromes:
Current Thinking on the Disease,
Diagnosis and Treatment
Rafael Bejar MD, PhD
Aplastic Anemia & MDS International Foundation
Regional Patient and Family Conference
April 5th, 2014
Overview
• Introduction to MDS
• Pathophysiology
• Clinical Practice
- Making the diagnosis
- Risk stratification
- Selecting therapy
• Future Directions/Challenges
Low Blood Counts
65 year-old woman with mild anemia and a platelet count
that fell slowly from 230 to 97 over the past 3 years.
Normal
Range
Myelodysplastic Syndromes
• Shared features:
– Ineffective differentiation and low blood counts
– Clonal expansion of abnormal cells
– Risk of transformation to acute leukemia
• Afflicts 15,000 – 45,000 people annually
• Incidence rises with age (mean age 71)
ASH Image Bank
MDS Incidence Rates 2000-2008
US SEER Cancer Registry Data
Incidence Rate per 100,000
60
50
40
30
20
10
0
35-39
40-44
45-49
50-54
55-59
60-64
Age
http://seer.cancer.gov. Accessed May 1, 2013.
65-69
70-74
75-79
80-84
>85
Age and Sex in MDS
 Overall incidence in this analysis: 3.4 per 100,000
36.4*
50
Overall
40
Males
Females
20.9
30
20
7.5
10
0
0.1
0.7
< 40
40-49
*P for trend < .05
Slide borrowed from Dr. David Steensma
2.0
50-59
60-69
Age at MDS diagnosis (years)
70-79
≥ 80
Rollison DE et al Blood 2008;112:45-52.
Etiology of MDS
85%
10-15%
<5%
“De novo”
(idiopathic, primary)
Ionizing radiation,
DNA alkylating agents
(chlorambucil, melphalan,
cyclophosphamide, etc.)
Topoisomerase II inhibitors
(etoposide, anthracyclines,
etc.)
Median age ~71 years;
increased risk with aging
Peaks 5-7 years
following exposure
Peaks 1-3 years
following exposure
Slide borrowed from Dr. David Steensma
Risk factors for MDS
Environmental
Inborn
AGING
Fanconi anemia
Exposure to DNA alkylating agents
(chlorambucil, melphalan,
cyclophosphamide)
Familial Platelet Disorder with AML
Predisposition (“FPD-AML”) (RUNX1,
CEBPA)
GATA2
mutant
Exposure to topoisomerase II inhibitors
(etoposide, anthracyclines)
Exposure to ionizing radiation
Environmental / occupational
exposures (hydrocarbons etc.)
Antecedent acquired
hematological disorders
Aplastic anemia (15-20%)
PNH (5-25%)
Slide borrowed from Dr. David Steensma
(MonoMACsyndrome: monocytopenia,
B/NK lymphopenia, atypical
mycobacteria and viral and other
infections, pulmonary proteinosis,
neoplasms)
Other congenital marrow failure
syndromes or DNA repair defects
(Bloom syndrome, ataxiatelangiectasia, etc.)
Familial syndromes of unknown origin
Corrupted Hematopoiesis
Differentiation
Normal
Transformation
Early
MDS
Advanced
MDS
Secondary AML
Making the Diagnosis
Diagnostic Overlap
Fanconi
Anemia
Acute Myeloid
Leukemia (AML)
Aplastic Anemia
Paroxysmal
Nocturnal
Hematuria
Myelodysplastic
Syndromes (MDS)
T-LGL
Myeloproliferative
Neoplasms
Myelodysplastic Syndromes
Minimum Evaluation Needed
Diagnosis of MDS is largely MORPHOLOGIC, so you need is:
Bone Marrow Aspirate/Biopsy
Complete Blood Count with white cell differential
Karyotype (chromosome analysis)
Sometimes useful:
MDS FISH panel – usually if karyotype fails
Flow cytometry – aberrant immunophenotype
Genetic Testing – may become standard eventually
Minimal Diagnostic Criteria
Cytopenia(s):
• Hb <11 g/dL, or
• ANC <1500/μL, or
• Platelets <100 x 109L
MDS “decisive” criteria:
• >10% dysplastic cells in 1 or more lineages,
or
• 5-19% blasts, or
• Abnormal karyotype typical for MDS, or
• Evidence of clonality (by FISH or another
test)
•
•
•
•
•
•
•
•
Other causes of cytopenias and morphological changes EXCLUDED:
Vitamin B12/folate deficiency
HIV or other viral infection
Copper deficiency
Alcohol abuse
Medications (esp. methotrexate, azathioprine, recent chemotherapy)
Autoimmune conditions (ITP, Felty syndrome, SLE etc.)
Congenital syndromes (Fanconi anemia etc.)
Other hematological disorders (aplastic anemia, LGL disorders, MPN etc.)
Valent P, et al. Leuk Res. 2007;31:727-736.
Slide borrowed from Dr. David Steensma
Valent P et al Leuk Res 2007;31:727-736.
Looking for Answers
65 year-old woman with mild anemia and a platelet count
that fell slowly from 230 to 97 over the past 3 years.
Normal
Range
B12 level - Normal
Folate
- Normal
Thyroid - Normal
No toxic medications
No alcohol use
No chronic illness
Bone Marrow Biopsy
65 year-old woman with mild anemia and a platelet count that fell
slowly from 230 to 97 over the past 3 years.
Too many cells in the bone marrow
No extra ‘blasts’ seen
Chromosomes are NORMAL
Classification of MDS Subtypes
World Health Organization MDS categories (2008)
Name
Refractory cytopenia with
unilineage dysplasia
(RCUD)
Refractory anemia with
ring sideroblasts
MDS associated with
isolated del(5q)
Refractory cytopenia with
multilineage dysplasia
Refractory anemia with
excess blasts, type 1
Refractory anemia with
excess blasts, type 2
MDS - unclassified
Abbreviation
Blood findings
Refractory anemia (RA)
Bone Marrow findings
•
•
•
Unicytopenia; occasionally
bicytopenia
No or rare blasts (<1%)
•
•
Anemia
No blasts
•
•
•
Anemia
Usually normal or increased
platelet count
No or rare blasts (<1%)
RCMD
•
•
•
•
Refractory neutropenia (RN)
•
•
Refractory thrombocytopenia (RT)
•
RARS
•
•
•
•
Unilineage dysplasia (≥10% of cells in one
myeloid lineage)
<5% blasts
<15% of erythroid precursors are ring
sideroblasts
≥15% of erythroid precursors are ring
sideroblasts
Erythroid dysplasia only
<5% blasts
•
•
Isolated 5q31 deletion
Normal to increased megakaryocytes with
hypolobated nuclei
<5% blasts
No Auer rods
Cytopenia(s)
No or rare blasts (<1%)
No Auer rods
<1 x 109/L monocytes
•
•
•
•
≥10% of cells in ≥2 myeloid lineages dysplastic
<5% blasts
No Auer rods
±15% ring sideroblasts
RAEB-1
•
•
•
•
Cytopenia(s)
<5% blasts
No Auer rods
<1 x 109/L monocytes
•
•
•
Unilineage or multilineage dysplasia
5-9% blasts
No Auer rods
RAEB-2
•
•
•
•
Cytopenia(s)
5-19% blasts
±Auer rods
<1 x 109/L monocytes
•
•
•
Unilineage or multilineage dysplasia
10-19% blasts
±Auer rods
•
MDS-U
•
•
Cytopenia(s)
≤1% blasts
Minimal dysplasia but clonal cytogenetic
abnormality considered presumptive evidence of
MDS
<5% blasts
Del(5q)
•
Swerdlow SH, Campo E, et al, eds. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, 4th edition.
Lyon: IARC Press, 2008, page 89 (Section: Brunning RD et al, “Myelodysplastic syndromes/neoplasms, overview)”.
World Health Organization MDS/MPN categories (2008)
Name
Refractory anemia with
ring sideroblasts and
thrombocytosis
Abbreviation
Blood findings
•
•
•
•
≥15% of erythroid precursors are ring
sideroblasts
Erythroid dysplasia only
<5% blasts
proliferation of large megakaryocytes
>1 x 109/L monocytes
<5% blasts
•
•
Unilineage or multilineage dysplasia
<10% blasts
•
•
>1 x 109/L monocytes
5%-19% blasts or Auer rods
•
•
Unilineage or multilineage dysplasia
10%-19% blasts or Auer rods
aCML
•
•
•
WBC > 13 x 109/L
Neutrophil precursors >10%
<20% blasts
•
•
•
Hypercellular
<20% blasts
BCR-ABL1 negative
JMML
•
•
>1 x 109/L monocytes
<20% blasts
•
•
•
Unilineage or multilineage dysplasia
<20% blasts
BCR-ABL1 negative
•
Dysplasia with myeloproliferative
features
No prior MDS or MPN
•
Dysplasia with myeloproliferative features
RARS-T
•
•
•
Anemia
No blasts
≥450 x 109/L platelets
Chronic myelomonocytic
leukemia, type 1
CMML-1
•
•
Chronic myelomonocytic
leukemia, type 2
CMML-2
Atypical chronic myeloid
leukemia
Juvenile myelomonocytic
leukemia
MDS/MPN – unclassified
(‘Overlap Syndrome’)
Bone Marrow findings
MDS/MPN-U
•
Swerdlow SH, Campo E, et al, eds. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, 4th edition.
Lyon: IARC Press, 2008, page 89 (Section: Brunning RD et al, “Myelodysplastic syndromes/neoplasms, overview)”.
Genetic Abnormalities in MDS
Translocations /
Rearrangements
Rare in MDS:
Uniparental disomy /
Microdeletions
Rare - often at sites of
point mutations:
Copy Number Change
Point Mutations
About 50% of cases:
Most common:
t(6;9)
4q
TET2
del(5q)
i(17q)
7q
EZH2
-7/del(7q)
t(1;7)
11q
CBL
del(20q)
t(3;?)
17p
TP53
del(17p)
t(11;?)
del(11q)
inv(3)
del(12p)
idic(X)(q13)
+8
-Y
Observed Frequency in MDS
Likely in all cases
~80% of cases have
mutations in a
known gene
Point Mutations in MDS
Tyrosine Kinase Pathway
JAK2
KRAS
BRAF
RUNX1
ETV6
NRAS
PTPN11
Transcription Factors
TP53
WT1
CBL
PHF6
BCOR
EZH2
ZRSF2
U2AF1
DNMT3A
SF3B1
PRPF40B
U2AF2
UTX
SRSF2
ASXL1
SF1
ATRX
CALR
BRCC3
GNAS/GNB1
Cohesins
Splicing Factors
SETBP1
TET2
NPM1
RTK’s
Epigenetic Dysregulation
IDH
1&2
GATA2
Others
PRPF8
SF3A1
Prognostic Risk Assessment
MDS Risk Assessment
65 year-old woman with mild anemia and a platelet count
that fell slowly from 230 to 97 over the past 3 years.
Normal
Range
Diagnosis:
Refractory cytopenia with
unilineage dysplasia
WHO Prognostic Scoring System
Malcovati et al. Haematologica. 2011;96:1433-40.
*Median survival ranges for the WPSS were estimated from Malcovati et al.
Haematologica. 2011 Oct;96(10):1433-40
International Prognostic Scoring System
Greenberg et al. Blood. 1997;89:2079-88.
IPSS-Revised (IPSS-R)
ipss-r.com
Greenberg et al. Blood. 2012:120:2454-65.
MDS Risk Assessment
65 year-old woman with mild anemia and a platelet count
that fell slowly from 230 to 97 over the past 3 years.
Normal
Range
Diagnosis:
Refractory cytopenia with
unilineage dysplasia
WPSS - Very Low Risk
IPSS - Low Risk
IPSS-R - Very Low Risk
Risk Adapted Therapy
Treatment Options for MDS
Observation
Erythropoiesis stimulating agents
Granulocyte colony stimulating factor
Iron chelation
Red blood cell transfusion
Platelet transfusion
Lenalidomide
Immune Suppression
Hypomethylating agent
Stem cell transplantation
Options
Clinical Trials – always the best option
MDS Risk Assessment
65 year-old woman with mild anemia and a platelet count
that fell slowly from 230 to 97 over the past 3 years.
Normal
Range
Diagnosis:
Refractory cytopenia with
unilineage dysplasia
WPSS - Very Low Risk
IPSS - Low Risk
IPSS-R - Very Low Risk
Treating Lower Risk MDS
Primary Goal: to improve QUALITY OF LIFE
1. Do I need to treat at all?
- No advantage to early aggressive treatment
- Observation is often the best approach
2. Are transfusions treatment?
- No! They are a sign that treatment is needed.
Guidelines for Lower Risk MDS
Primary Goal: to improve QUALITY OF LIFE
Treating Lower Risk MDS
Primary Goal: to improve QUALITY OF LIFE
What if treatment is needed?
1. Is my most effective therapy likely to work?
- Lenalidomide (Revlimid)
In del(5q) – response rates are high
50%-70% respond to treatment
Median 2-years transfusion free!
Treating Lower Risk MDS
Primary Goal: to improve QUALITY OF LIFE
Is my second most effective therapy likely to work?
- Red blood cell growth factors
- Erythropoiesis Stimulating Agents (ESAs)
- Darbepoetin alfa (Aranesp)
- Epoetin alfa (Procrit, Epogen)
- Lance Armstrong Juice  EPO
Erythropoiesis Stimulating Agents
Primary Goal: to improve QUALITY OF LIFE
ESAs
TPO mimetics
G-CSF (neupogen)
ESAs – act like our own erythropoietin
Serum EPO level (U/L)
<100
RBC transfusion requirement
= +2 pts
100-500 = +1 pt
>500
<2 Units / month = +2 pts
≥2 Units / month = -2 pts
= -3 pts
Total Score
Response Rate
High likelihood of response: > +1
74% (n=34)
Intermediate likelihood: -1 to +1
23% (n=31)
Low likelihood of response: < -1
7% (n=39)
Hellstrom-Lindberg E et al Br J Haem 2003; 120:1037
Growth Factor Combinations
Primary Goal: to improve QUALITY OF LIFE
ESAs
TPO mimetics
G-CSF (neupogen)
ESAs can be combined with G-CSF
- response rate of 46.6%, EPO <200 and <5% blasts predictive
ESAs can be combined with Lenalidomide
- response rate of 31% to Len, 52% to both. TI 18.4% vs. 32.0%!
ESAs can be combined with Azacitidine – not yet standard
Greenberg, P. L., Z. Sun, et al. (2009) Blood 114(12): 2393-2400.
Toma A et al (ASCO Abstract) J Clin Oncol 31, 2013 (suppl; abstr 7002)
Thrombopoietin Mimetics
Primary Goal: to improve QUALITY OF LIFE
ESAs
TPO mimetics
G-CSF (neupogen)
Eltrombopag and Romiplostim - approved, but not in MDS
Initial concern about increasing blasts and risk of AML
Follow-up suggests Romiplostim safe in lower risk patients
Mittleman M et al ASH Abstracts, 2013. Abstract #3822
Kantarjian H et al ASH Abstracts, 2013. Abstract #421
Treating Lower Risk MDS
Primary Goal: to improve QUALITY OF LIFE
What my next most effective therapy?
- Immunosuppression
Some MDS patients have features of aplastic anemia
- Hypoplastic bone marrow (too few cells)
- PNH clones
- Certain immune receptor types (HLA-DR15)
Immune Suppression for MDS
Primary Goal: to improve QUALITY OF LIFE
Swiss/German Phase III RCT of ATG + Cyclosporin (88 patients)
Mostly men with Lower Risk MDS
CR+PR: 29% vs. 9%
No effect on survival
Predictors of Response:
- hypocellular aspirate
- lower aspirate blast %
- younger age
- more recent diagnosis
Passweg, J. R., A. A. N. Giagounidis, et al. (2011). JCO 29(3): 303-309.
Hypomethylating Agents
Inhibitors of DNA methyl transferases:
Iron Balance and Transfusions
Daily intake
1.5 mg (0.04%)
Tightly regulated
Daily losses only
1.5 mg (0.04%)
Not regulated!
Every three
units of blood
3-4 grams of Iron
in the body
What About Iron Chelation?
More transfusions and elevated ferritin levels are associated
with poor outcomes in MDS patients.
Are these drivers of prognosis or just reflective of disease?
Retrospective studies suggest survival advantage!
small prospective and large population based Medicare studies show
survival benefit, INCLUDING hematologic responses (11-19%).
I consider treatment in lower risk, transfusion dependent
patients with long life expectancy after 20+ transfusions.
Zeidan et al. ASH Meeting. 2012. Abstract #426.
Nolte et al. Ann Hematol. 2013. 92(2):191-8.
How to Chelate Iron
Three ways are FDA approved:
Deferoxamine (Desferal) – subcutaneous pump 8-12 hrs/day
Deferasirox (Exjade) – oral suspension – once per day
Deferiprone (Ferriprox) – oral pill form – 3x per day
But side effects and adverse events can be significant!
Deferasirox – renal, hepatic failure and GI bleeding
Deferiprone – agranulocytosis (no neutrophils!)
Guidelines for Lower Risk MDS
Primary Goal: to improve QUALITY OF LIFE
1. Do I need to treat?
- symptomatic cytopenias
2. Is LEN likely to work?
- del(5q) ±
3. Are ESA likely to work? - Serum EPO < 500
4. Is IST likely to work?
- hypocellular, DR15, PNH
5. Think about iron!
- 20 or more transfusions
6. Consider AZA/DEC
7. Consider HSCT or clinical trial!
Guidelines for Lower Risk MDS
Special Considerations:
Transfusion Dependence
- Indication for treatment – even with AZA/DEC, consider chelation
Del(5q)
- High response rate to LEN even if other abnormalities
Serum EPO level
- Used to predict EPO response, > 500  unlikely to work
Indication for G-CSF
- used to boost EPO, not for primary neutropenia
Immunosuppresive Therapy
- ≤ 60y, hypocellular marrow, HLA-DR15+, PNH clone
Future Directions
Limitations of the IPSS/IPSS-R
 Less than half of patients have relevant cytogenetic
abnormalities
 Heterogeneity remains within each risk category,
particularly the lower-risk categories
 Excludes therapy related disease and CMML
 Is only validated at the time of initial diagnosis in
untreated patients
The IPSS’s do not include molecular abnormalities
Mutation Frequency and Distribution
Complex (3 or more abnormalities)
Bejar et al. NEJM. 2011;364:2496-506.
Bejar et al. JCO. 2012;30:3376-82.
TP53 Mutations and Complex Karyotypes
TP53 Mutated
Complex Karyotype
The adverse prognostic impact of the complex karyotype is entirely
driven by its frequent association with mutations of TP53
Impact of Mutations by IPSS Group
1.0
1.0
0.9
TP53
Overall Survival
ETV6
0.8
0.7
0.6
0.5
0.4
0.3
0.8
0.5
0.4
0.3
0.1
1
2
3
4
5
6
7
8
0.0
9 10 11 12 13
0
1
2
3
4
5
IPSS Int1 Mut Absent (n=128)
IPSS Int1 Mut Present (n=57)
p < 0.001
IPSS Int2 (n=101)
8
9 10 11 12 13
0.7
0.6
0.5
0.4
0.3
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.2
0.1
0.1
0
1
2
Bejar et al. NEJM. 2011;364:2496-506.
3
4
5
6
Years
7
8
9 10 11 12 13
IPSS Int2 Mut Absent (n=61)
IPSS Int2 Mut Present (n=40)
p = 0.02
IPSS High (n=32)
0.9
Overall Survival
Overall Survival
7
1.0
0.8
0.0
6
Years
Years
0.9
RUNX1
0.6
0.1
1.0
EZH2
0.7
0.2
0
IPSSIPSS
LowLow
Mut(n=110)
Absent (n=87)
IPSS Low Mut Present (n=23)
p < 0.001
IPSS Int1 (n=185)
0.9
0.2
0.0
ASXL1
(n=110)
(n=185)
(n=101)
(n=32)
Overall Survival
IPSS Low
IPSS Int1
IPSS Int2
IPSS High
0.0
0
1
2
3
4
5
6
Years
7
8
9 10 11 12 13
Tracking the Founder Clone
Walter MJ et al. NEJM 2012;366(12):1090-8.
Clonal Evolution
Walter MJ et al. NEJM 2012;366(12):1090-8.
Clinical Sequencing and Banking
Targeted Massively Parallel Sequencing
Clinical
Information
Viable Cells
Tumor DNA/RNA
Germline DNA
Biorepository
Extensive
Genotypic
Annotation
Acknowledgements:
Bejar Lab - UCSD
Columbia University
Albert Perez
Azra Raza
Brigham and Women’s
MD Anderson Cancer Center
Ben Ebert
Allegra Lord
Ann Mullally
Anu Narla
Bennett Caughey
Bernd Boidol
Damien Wilpitz
Marie McConkey
Guillermo Garcia-Manero
Hagop Kantarjian
Sherry Pierce
Gautam Borthakur
DFCI / Broad
David Steensma
Donna Neuberg
Kristen Stevenson
Mike Makrigiorgos
Derek Murphy
Naomi Galili
Memorial Sloan-Kettering
Ross Levine
Omar Abdel-Wahab