Transcript The secondary antibody

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Final Jeopardy
Medical
Serial
ELISA
Interventions Dilutions
Outbreaks
Immune
System
Vocabulary
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What does ELISA
stand for?
Enzyme-Linked
Immunosorbant
Serological Assay
What are the 2
types of ELISA
tests?
Antigen Detection
ELISA
&
Antibody Detection
ELISA
What is
significant about
the secondary
antibody created
for the ELISA
test?
The secondary antibody:
• Is created to
specifically bind the
primary antibody
• It is conjugated with
an enzyme
What reaction
occurs in an
ELISA, causing a
visible color
change?
The enzyme
oxidizes the
substrate?
Why are antibodies
used to identify the
presence of a
particular antigen?
Antibodies are only
created in response
to a specific
antigen and will
only bind that one
antigen
What is a medical
intervention?
Any measure that
improves health or
alters the course of
a disease.
List 3
interventions that
could be
documented for
Sue Smith
Inhaler
Ibuprofen
Pathogen DNA sequencing
ELISA
Urine, blood or lymph samples
Antibiotics for N. meningitidis
List 3 major
medical
intervention
categories
Diagnostics
Surgery
Medications
rehabilitation
Genetics
Immunology
Medical devices
Which medical
intervention
category does an
ELISA fall into?
diagnostics
List 3 interventions
that could be used
for a broken leg.
Xray
Pain meds
Cast
Crutches/wheel chair
surgery
What is the liquid
used to dilute a
substance called?
diluent
How is a tube
dilution calculated?
Amount of sample
_________________
Total volume in
tube
Calculate the tube
dilution for the
following tube:
1/10
Calculate the final
dilution for tube #4
1/
10,000
If tube #1 has a
concentration of 100
ug/ml, what is the
concentration in tube #3?
1
ug/ml
What is an
outbreak?
A rise in disease
occurrence in an
area
List 4 steps taken
by scientists during
an outbreak
Interview patients
Symptoms tracker
Patient connections web
Pathogen DNA sequencing
ELISA
Treat
Stop spread
Prevent future spread
What is the best
medical
intervention to
determine patient
zero?
Quantitative
concentration data
from an ELISA.
How do scientists
determine the
pathogen
responsible for the
outbreak?
Pathogen DNA
isolation and
sequencing
Why is it important for
doctors to know the
concentration of
pathogen in patients
during an outbreak?
2 reasons
1. Determine
patient zero
2. Track the path of
infection
Which immune
system structure
produces
antibodies?
B cells
How many antigens
will an antibody
bind?
Only one
What is an
antigen?
An identifying
protein marker on
the surface of cells
What does the
presence of the
specific antibody
for HIV in a
patient’s system
indicate?
That patient has
encountered the
HIV virus in their
body
Why is antigen
concentration alone
sometimes not
enough to
determine patient
zero?
A patient’s immune
system may have
already reduced the
amount of pathogen
present
What is the collection,
classification, storage, and
analysis of biochemical and
biological information using
computers especially as applied
in molecular genetics and
genomics
Bioinformatics
What is a specific
causative agent of
disease?
Pathogen
The amount of a
specified substance
in a unit amount of
another substance
concentration
What is an antigen-binding
immunoglobulin, produced
by B cells, that functions as
the effector in an immune
response
Antibody
What is a foreign
macromolecule (protein) that
does not belong to the host
organism and elicits an
immune response?
An antigen
Make your wager
How does a visible color
change in an antigen
detection ELISA prove
the presence of the
antigen?
The enzyme connected to the
secondary antibody oxidizes the
substrate to produce a color change.
The secondary antibody is not present
unless the primary antibody is present.
The primary antibody is not present
unless the antigen is present.
Therefore, the presence of the
secondary antibody proves the
presence of the antigen.