Transcript document

Dendritic cells and mast cells :
their role in the progression of the Mycosis
Fungoides
Salvatore Marasà ¹,Daniela Cabibi¹, Lorenzo Marasà ²,Federico Aragona¹.
1 Institute of Pathologic Anatomy, University of Palermo, Italy.
2 C.O.U. of Pathologic Anatomy, ARNAS-Civico Hospital, Palermo, Italy.
Mycosis fungoides (MF) is a special type of cancer, in
which the neoplastic cells and those responsive to
them are represented by the same cell: lymphocytes.
The prolonged course of disease and the bystander
intense infiltrate suggest a strong antineoplastic
immune response.
Progression of the Mycosis Fungoides
PATCH STAGE
PLAQUE STAGE
TUMOUR STAGE
Aims
We studied the role of the epidermal Langherans
cells(LCs), dermal dendritic cells (DDCs) and the mast
cells (MCs) in the progression of the MF.
Materials and methods
The study included 32 patients.
The immunoreactivity was investigated quantitatively
and topographically using monoclonal antibodies
CD3,CD4,CD8,CD1a,Lag,Langerina,S100,Tryptase,
cKit,CD31,CD34,FactorVIII,VEGF.
The average number of positive cells was calculated
for 5 fields with higher cell density at 400x.
The intensity of cell immunostaining was scored as
0(0-5 cells),1 (5-10 cells) and 2 (+10cells).
The neovascularisation was studied using MAGS and
Microvessel Density.
Results
LCs were significantly increased in plaque stage;
conversely during tumour stage they decreased
to the point that microascessi were almost free.
To compensate for this reduction, however,
during the nodular period, we noted an increase
in the population of DDCs.
MCs and neoangiogenesis was accentuated in
tandem with the progression of MF.
Epidermal Langherans Cells (LCs), Dermal Dendritic Cells (DDCs )CD1a+
Patch Stage
Tumour stage
Neovascularisation
Patch stage
Neovascularisation and Mast Cells (MCs)
Tumour stage
Plaque stage
E.E.
Tryptase
Conclusions
This study support the dual role of LCs and
DDCs in the MF: in the early stages they are
involved in the host defense against
malignant T lymphocytes, while in advanced
stages they are involved in a immune fall of
the host to which they themselves would be
involved. Besides the neovascularization
closely is correlated with tumour progression and amount of perivascular tryptasepositive MCs .
References
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3.Marasà L.et al.: Pathologica1983,75:353-365.
4.Weidner N. et al.: N Engl J Med 1991,324 : 1-8.