No Slide Title - University of Windsor
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Transcript No Slide Title - University of Windsor
INTRODUCTION TO
PLATELETS
developed by
Liz Fracalanza
Tina Goodyear
Hugh B. Fackrell
Dept of Biological Sciences
University of Windsor
Windsor, ON Canada
OBJECTIVES
To examine the formed
elements of blood
To define the structure and
function of the cellular
elements
To differentiate between
the various blood cells
Introduction
Blood may be separated
into a clear fluid
composed of either serum
or plasma and formed
elements which contain
cells and cell fragments
Formed Elements of Blood
Thrombocytes (platelets)
Erythrocytes (red blood cells)
Leucocytes (white blood cells)
Prepartion of Blood
Prevent coagulation by the
addition of an anticoagulant
Smear blood onto a glass slide,
stain and examine under a
microscope
Use an oil immersion or a highdry objective
Use an area of the slide with
little red cell overlap
PLATELETS
Platelet Characteristics
Diameter
:2.9 - 4.3 um
Thickness :0.7 - 1.1 um
Cytoplasm :purple/blue-grey
Nucleus
:none
Life-span :8 - 12 days
Granules :Present(not visible)
Platelet Function
Maintain blood vessel integrity
Prevent blood loss to maintain
hemostasis
Synthesize chemical substances
provide a reaction surface for
the coagulation cascade
Antibody - A specific immunoglobulin
(protein) produced by plasma cells
or B cells in response to a foreign
particle.
Anticoagulant - A substance that
inhibits blood clotting.
Basophilic - To have an affinity for
basic dyes such as methylene blue
used in the Wright stain.
Chemotaxis - A chemical
stimulation causing the movement
of a om the chemical source. It
is a part of the inflammation
response.
Chromatin - The stainable
material of the cell nucleus
composed of basic histones,
condensed nucleoprotein and
parachromatin
Coagulation - The series of chemical
reactions that result in blood
clotting.
Complement - Serum proteins that
participate in an enzymatic
cascade, resulting in a cytolytic
membrane attack complex.
Endothelial Lining - A layer of
cells that line the blood and
lymphatic vessels.
Hemostasis - An equilibrium of
blood cells and plasma required
to support life (homeostasis).
Heparin - An anticoagulant used to
inhibit the coagulation cascade by
enhancing the activity of antithrombin III.
Histamine - A vasoactive amine
secreted by the blood basophils
and mast cells when stimulated by
IgE antibodies.
Lymphokine - A protein released by
lymphocytes that acts as a
cellular mediator in the immune
response.