Transcript 1.6 PPT_Parts of the Microscope

Objective
• We will be able to identify part of a
microscope (Goal 1).
Parts of the Microscope
and Their Function
On the next slide there is an
image of a microscope, very
similar to the scopes we use in
class.
Click on the boxes to see the
name and function of each part of
the microscope.
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#9 Ocular Lense—The part
you look at with your eye.
Usually 10 X magnification.
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#10 Arm – Used to safely
transport microscope
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#11 Stage – Slides are
placed on this
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#12 Coarse Adjustment –
Used to make large changes
in focus. NOTE Never use this
when viewing on high power
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#13 Fine Adjustment –
Used to make small
adjustments of focus
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#14 Base – Used to safely
transport the microscope
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#1 Tube – Reflects light up
to the viewers eye
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#2 Revolving Nosepiece –
Allows for quick change of
objectives
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#3 Low Power Objective –
The first lens you use when
doing proper microscope
work. Usually 4 X
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#4 Medium Power
Objective – The second
lens you use when doing
proper microscope work.
Usually 10 X
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#5 High Power Objective – The
highest magnification used.
Usually 43 X. NEVER use the
course adjustment when using
this lens.
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#6 Stage Clips – Use to
keep the slide in place.
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#7 Diaphragm – Use to vary the
amount of light passing through
the slide. Usually it is better if
the amount of light is low.
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#8 Light Source – Sends
light up through the
diaphragm and through the
slide for viewing
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Images Produced by Light
Microscopes
Amoeba
Streptococcus bacteria
Anthrax bacteria
Human cheek cells
Plant cells
Yeast cells
Microscope Magnification
• The ocular lens has a magnification of 10x
• The objective lenses have magnifications of:
– Low: 4x
– Medium: 10x
– High: 40x
• To find the total magnification: Ocular lens x Objective lens
– Example: ocular lens x high power = total magnification
10 x 40 = 400 (total magnification)
How to Carry a Microscope
• ALWAYS carry with the ARM and BASE
REMINDERS
• REMEMBER: All specimens must be thin for LIGHT to shine
through them, otherwise they cannot be viewed clearly.
• REMEMBER: DO NOT use the COARSE adjustment knob when
focusing on medium and high power objectives!! This could
damage the objective and possibly break the slides and cover slips.
If you cannot get it in focus, you should use the FINE
ADJUSTMENT KNOB.