Transcript The Compound Microscope

Parts of the Microscope
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Base: supports microscope
 Carry with one hand supporting
the base!
Arm: connects base and body tube
 Carry with one hand supporting
the arm!
Ocular lens: eyepiece (10x)
Body tube: supports nosepiece w/
objective lenses
 Objective lenses:
 Scanning lens (4x) RED
 Low power (10x) YELLOW
 High power (40x) BLUE
 Oil immersion (100x) WHITE
Parts of the Microscope
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Nosepiece:
 moveable
 carries 3 objective lenses
Stage:
platform for slide
 has a light hole
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Diaphram: regulates amount
of light
Fine adjustment knob: small
knob
Coarse adjustment knob: large
knob
The Compound Light
Microscope
How does a compound light
microscope work?
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A compound microscope
uses multiple lenses to
increase total magnification.
The term light refers to the
method by which light
transmits the image to your
eye. Compound deals with the
microscope having more
than one lens. Microscope is
the combination of two
words; "micro" meaning
small and "scope" meaning
view.
Field of View
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The total area visible through the ocular lens.
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The higher the magnification, the smaller the field
of view.
Scanning: 40x
Low Power: 100x
High Power: 400x
Calculating total magnification
Total Magnification = ocular lens x objective lens
For example, if the 40x objective is in place the
400x (10*40=400)
total magnification would be _______________.
Drawings
1.
R.B.C
Don’t even think of starting
your drawing unless you have
a PENCIL!
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Pen is UNACCEPTABLE!
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This is for two reasons:
(a) You can erase pencil!
(b) You can shade in
areas more easily in pencil.
Liver cells
W.B.C
Blood Cells
Mag: 100x
Drawings
Draw a circle for each
slide you will be viewing
(about the size of a petri
dish). You must
include clear, proper
labels including the
specimen name as
written on the slide
and the magnification.
2.
R.B.C
W.B.C
Blood Cells
Mag: 100x
Compact bone
Magnification: 400x
Drawings
R.B.C
3.
Labels should start on the
outside of the circle.
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The circle indicates the
field of view as seen
through the eyepiece.
All arrows should end with
the point touching the
object to be labeled!
W.B.C
Blood Cells
Mag: 100x
Focusing the Microscope
1.
2.
3.
4.
5.
6.
Locate the slide material at scanning power (4x).
Then move the area to be viewed to the center of the
field of view.
Make sure the slide is in perfect focus at the lower
objective before moving up to a higher objective.
Turn your objective without moving the slide or stage.
Slightly adjust the image at low power (100x) with the
fine adjustment knob only for perfect focus.
Turn your objective to high power (400x), and use only
the fine adjustment knob and do not turn it more than
1/2 turn.
Rules to Remember
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Always carry with one hand on arm and one on
base of the microscope.
Use a slide and cover-slip.
Clean Up:
 Always return to scanning (lowest) power.
 Always lower stage all the way.
 Unplug, wrap cord and cover.
SEM images