Transcript Microscope Parts

Understanding the
Microscope
Honors Biology
Compound Microscope
• Contains a
combination of two
lenses
Eyepiece or ocular lens
• Lens closest to the
eye
Objective Lens
• Lens closest to the
specimen or object
How to figure out magnification
• Eyepiece
•
10x
•
10x
•
10x
X
X
X
X
objective
=
Magnification
Nosepiece
• Objectives fastened
to this revolving
structure
Stage
• Where you place
your slide
Stage clips
• Hold slide in place
Body Tube
• Light travels through
here
Light Source
• Provides light
Diaphragm
• Regulates the light
reaching the
objective lens
Base
• Supports the
microscope
Arm
• Holds the body
tube, acts as a
handle
Coarse focusing knob
• Used to focus under
low power only
Fine focusing knob
• Used to focus under
high power only
A microscope reverses and inverts the
image of an object seen under it
Before
viewing
under the
microscope
e
While
viewing
under the
microscope
e
Things moving under a microscope are
actually moving in the opposite direction
Things moving under a microscope are
actually moving in the opposite direction
Stereomicroscope
• Binocular
microscope
• Object is seen in
3-D
• Only used to see
large objects
• Does not reverse or
invert images
Transmission Electron Microscope
• Uses a beam of
electrons instead of light
rays
• 200,000x magnification
• tissues have to be sliced
really thin, dry and in a
vacuum chamber
• can’t be used with living
material
Scanning Electron Microscope
• provides images with 3-D quality
• can’t be used with living material
Micromanipulator
• Used to dissect
cells, can remove
nuclei
• Knowledge of cells
has been increased
by manipulation and
dissection of cells
High – Speed Centrifuge
• Spin at high speeds
• Cell components sort out at
different layers according
to density
Microscopic Measurements
• Use the unit called the
micrometer
• 1/1000 of a meter
How to convert millimeters to
micrometers
• Multiply by one thousand…or
• Move the decimal point 3 places to
the right